Main Article Content
Abstract
To investigate the effects of different hormonal treatments on the oocyte maturation in wild- caught grey mullet (Mugil cephalus), fish were subjected to 4 different hormonal treatments as follows: (a) a control group received 0.5 ml/kg B.W. physiological saline, (b) fish injected with 20 mg/kg carp pituitary extract (CPE) as a priming dose followed by 200 µg/kg sGnRHa as resolving dose, (c) fish injected with 200 µg/kg sGnRHa as a priming dose followed by the same injection as a resolving dose and, (d) fish received 20 mg/kg CPE as a priming dose followed by injection of 200 µg/kg sGnRHa in Freund’s incomplete adjuvant emulsion. All GnRHa treated groups received 20 mg/kg B.W. metoclopramide as dopamine antagonist (DA). The interval between two injections was 24 hrs. At 6, 12, 24 and 48 hours post final injection ovarian samples were taken by soft plastic catheter and the maturation of oocytes was examined according to oocyte diameter, position of germinal vesicle and coalescence of oil droplets. The results showed that all hormonal treatments were significantly effective in progression of the oocyte maturation in comparison to control group. The treatments (b) and (d) were more effective than treatment (c) and resulted in final oocyte maturation and germinal break down. It was for the first time that emulsified GnRHa (sGnRHa-FIA) was shown to be effective in final oocyte maturation and ovulation of the species. The results showed that all hormonal treatments were effective in induction of oocyte maturation in wild-caught grey mullet and could be used in commercial hatcheries.
Keywords
Wild grey mullet
Hormone
GnRHa
Oocyte maturation
Oman Sea.
Article Details
License
The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
How to Cite
VAZIRZADEH, A., & EZHDEHAKOSHPOUR, A. (2015). The effects of different hormonal treatments on the oocyte maturation in wild grey mullet (Mugil cephalus) collected from the Iranian coastal waters of the Oman Sea. Iranian Journal of Ichthyology, 1(1), 17–22. https://doi.org/10.22034/iji.v1i1.48
References
-
Abraham, M.; Blanc, N. & Yashouv, A. 1966. Oogenesis in five species of grey mullets (Teleostei, Mugilidae) from natural and landlocked habitats. Journal of Zoology 15(3-4): 155-172.
Aizen, J.; Meiri, I.; Tzchori, I.; Levavi-Sivan, B. & Rosenfeld, H. 2005. Enhancing spawning in the grey mullet (Mugil cephalus) by removal of dopaminergic inhibition. General and comparative endocrinology 142(1): 212-221.
El-Gharabawy, M.M. & Assem, S.S. 2006. Spawning induction in the Mediterranean grey mullet (Mugil cephalus) and larval developmental stages. African Journal of Biotechnology 5(19): 1836-1845.
FAO 2012. The State of World Fisheries and Aquaculture. (SOFIA). Rome: Food and Agriculture Organization of the United Nations 1-230.
Kuo, C.M.; Shehadeh, Z.H. & Milken, K.K. 1973. A preliminary report on the development, growth and survival of laboratory reared larvae of the grey mullet, (Mugil cephalus) L. Journal of Fish Biology 5(4): 459-470.
Lee, C.S. & Tamaru, C.S. 1988. Advances and future prospects of controlled maturation and spawning of grey mullet (Mugil cephalus) in captivity. Aquaculture 74(1): 63-73.
Lee, C.S.; Tamaru, C.S. & Kelley, C.D. 1988. The cost and effectiveness of CPH, HCG and LHRH-a on the induced spawning of grey mullet (Mugil cephalus). Aquaculture 73(1): 341-347.
Lee, C.S.; Tamaru, C.S.; Miyamoto, G. T. & Kelley, C.D. 1987. Induced spawning of grey mullet (Mugil cephalus) by LHRH-a. Aquaculture 62(3): 327-336.
Levavi-Zermonsky B. & Yaron, Z. 1986. Changes in gonadotropin and ovarian steroids associated with oocytes maturation during spawning induction in the carp. General and Comparative Endocrinology 62: 89-98.
Mirhashem Rostami, S.A.; Amini, K.; Joorjani, M.; Ghezel, H.C. & Shafiee, A. 2006. An investigation on artificial reproduction of Mugil cephalus. Iranian Scientific Fisheries Journal 14 (4): 181-196. (In Farsi, Abstract in English).
Monbrison, D.D.; Tzchori, I.; Holland, M.C.; Zohar, Y.; Yaron, Z. & Elizur, A. 1997. Acceleration of gonadal development and spawning induction in the Mediterranean grey mullet, (Mugil cephalus): Preliminary studies. Bamidjeh 49(4): 214-221.
Mylonas, C.C. & Zohar, Y. 2001. Use of GnRHa-delivery systems for the control of reproduction in fish. Reviews in Fish Biology and Fisheries 10(4): 463-491.
Samira, S.A.; Alaa, A. & Mona, M.M. 2008. Reproductive biology (histological & ultrastructure) and biochemical studies in ovaries of (Mugil cephalus) from Mediterranean water. Journal of Arabian Aquaculture Society 3(1): 1- 26.
Tamaru, C.S.; Kelley, C.D.; Lee, C.S.; Aida, K., & Hanyu, I. 1989. Effects of chronic LHRH-a + 17-methyltestosterone or LHRHa + testosterone therapy on oocyte growth in the striped mullet Mugil cephalus). General and comparative endocrinology 76(1): 114-127.
Tamaru, C.S.; Lee, C.S.; Kelley, C.D.; Miyamoto, G. & Moriwake, A. 1994. Oocyte growth in the striped mullet Mugil cephalus L. maturing at different salinities. Journal of the World Aquaculture Society 25(1): 109-115.
Vazirzadeh, A.; Hajimoradloo, A.; Esmaeili, H. R.; & Akhlaghi, M. 2008. Effects of emulsified versus saline administration of GnRHa on induction of ovulation in rainbow trout, Oncorhynchus mykiss. Aquaculture 280(1): 267-269.
Vazirzadeh, A.; Mojazi Amiri, B.; Yelghi, S.; Hajimoradloo, A.; Nematollahi, M.A. & Mylonas, C.C. 2011. Comparison of the effects of different methods of mammalian and salmon GnRHa administration on spawning performance in wild-caught female carp (Cyprinus carpio carpio) from the Caspian Sea. Aquaculture 320(1): 123-128.
Yousif, O.M.; Fatah, A.A.; Krishna, K.; Minh, D.V. & Hung, B.V. 2010. Induced spawning and larviculture of grey mullet, Mugil cephalus (Linnaeus 1758) in the Emirate of Abu Dhabi. Aquaculture Asia 15(1): 1-3.
Zohar, Y. & Mylonas, C.C. 2001. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture 197(1): 99-136.
References
Abraham, M.; Blanc, N. & Yashouv, A. 1966. Oogenesis in five species of grey mullets (Teleostei, Mugilidae) from natural and landlocked habitats. Journal of Zoology 15(3-4): 155-172.
Aizen, J.; Meiri, I.; Tzchori, I.; Levavi-Sivan, B. & Rosenfeld, H. 2005. Enhancing spawning in the grey mullet (Mugil cephalus) by removal of dopaminergic inhibition. General and comparative endocrinology 142(1): 212-221.
El-Gharabawy, M.M. & Assem, S.S. 2006. Spawning induction in the Mediterranean grey mullet (Mugil cephalus) and larval developmental stages. African Journal of Biotechnology 5(19): 1836-1845.
FAO 2012. The State of World Fisheries and Aquaculture. (SOFIA). Rome: Food and Agriculture Organization of the United Nations 1-230.
Kuo, C.M.; Shehadeh, Z.H. & Milken, K.K. 1973. A preliminary report on the development, growth and survival of laboratory reared larvae of the grey mullet, (Mugil cephalus) L. Journal of Fish Biology 5(4): 459-470.
Lee, C.S. & Tamaru, C.S. 1988. Advances and future prospects of controlled maturation and spawning of grey mullet (Mugil cephalus) in captivity. Aquaculture 74(1): 63-73.
Lee, C.S.; Tamaru, C.S. & Kelley, C.D. 1988. The cost and effectiveness of CPH, HCG and LHRH-a on the induced spawning of grey mullet (Mugil cephalus). Aquaculture 73(1): 341-347.
Lee, C.S.; Tamaru, C.S.; Miyamoto, G. T. & Kelley, C.D. 1987. Induced spawning of grey mullet (Mugil cephalus) by LHRH-a. Aquaculture 62(3): 327-336.
Levavi-Zermonsky B. & Yaron, Z. 1986. Changes in gonadotropin and ovarian steroids associated with oocytes maturation during spawning induction in the carp. General and Comparative Endocrinology 62: 89-98.
Mirhashem Rostami, S.A.; Amini, K.; Joorjani, M.; Ghezel, H.C. & Shafiee, A. 2006. An investigation on artificial reproduction of Mugil cephalus. Iranian Scientific Fisheries Journal 14 (4): 181-196. (In Farsi, Abstract in English).
Monbrison, D.D.; Tzchori, I.; Holland, M.C.; Zohar, Y.; Yaron, Z. & Elizur, A. 1997. Acceleration of gonadal development and spawning induction in the Mediterranean grey mullet, (Mugil cephalus): Preliminary studies. Bamidjeh 49(4): 214-221.
Mylonas, C.C. & Zohar, Y. 2001. Use of GnRHa-delivery systems for the control of reproduction in fish. Reviews in Fish Biology and Fisheries 10(4): 463-491.
Samira, S.A.; Alaa, A. & Mona, M.M. 2008. Reproductive biology (histological & ultrastructure) and biochemical studies in ovaries of (Mugil cephalus) from Mediterranean water. Journal of Arabian Aquaculture Society 3(1): 1- 26.
Tamaru, C.S.; Kelley, C.D.; Lee, C.S.; Aida, K., & Hanyu, I. 1989. Effects of chronic LHRH-a + 17-methyltestosterone or LHRHa + testosterone therapy on oocyte growth in the striped mullet Mugil cephalus). General and comparative endocrinology 76(1): 114-127.
Tamaru, C.S.; Lee, C.S.; Kelley, C.D.; Miyamoto, G. & Moriwake, A. 1994. Oocyte growth in the striped mullet Mugil cephalus L. maturing at different salinities. Journal of the World Aquaculture Society 25(1): 109-115.
Vazirzadeh, A.; Hajimoradloo, A.; Esmaeili, H. R.; & Akhlaghi, M. 2008. Effects of emulsified versus saline administration of GnRHa on induction of ovulation in rainbow trout, Oncorhynchus mykiss. Aquaculture 280(1): 267-269.
Vazirzadeh, A.; Mojazi Amiri, B.; Yelghi, S.; Hajimoradloo, A.; Nematollahi, M.A. & Mylonas, C.C. 2011. Comparison of the effects of different methods of mammalian and salmon GnRHa administration on spawning performance in wild-caught female carp (Cyprinus carpio carpio) from the Caspian Sea. Aquaculture 320(1): 123-128.
Yousif, O.M.; Fatah, A.A.; Krishna, K.; Minh, D.V. & Hung, B.V. 2010. Induced spawning and larviculture of grey mullet, Mugil cephalus (Linnaeus 1758) in the Emirate of Abu Dhabi. Aquaculture Asia 15(1): 1-3.
Zohar, Y. & Mylonas, C.C. 2001. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture 197(1): 99-136.
Aizen, J.; Meiri, I.; Tzchori, I.; Levavi-Sivan, B. & Rosenfeld, H. 2005. Enhancing spawning in the grey mullet (Mugil cephalus) by removal of dopaminergic inhibition. General and comparative endocrinology 142(1): 212-221.
El-Gharabawy, M.M. & Assem, S.S. 2006. Spawning induction in the Mediterranean grey mullet (Mugil cephalus) and larval developmental stages. African Journal of Biotechnology 5(19): 1836-1845.
FAO 2012. The State of World Fisheries and Aquaculture. (SOFIA). Rome: Food and Agriculture Organization of the United Nations 1-230.
Kuo, C.M.; Shehadeh, Z.H. & Milken, K.K. 1973. A preliminary report on the development, growth and survival of laboratory reared larvae of the grey mullet, (Mugil cephalus) L. Journal of Fish Biology 5(4): 459-470.
Lee, C.S. & Tamaru, C.S. 1988. Advances and future prospects of controlled maturation and spawning of grey mullet (Mugil cephalus) in captivity. Aquaculture 74(1): 63-73.
Lee, C.S.; Tamaru, C.S. & Kelley, C.D. 1988. The cost and effectiveness of CPH, HCG and LHRH-a on the induced spawning of grey mullet (Mugil cephalus). Aquaculture 73(1): 341-347.
Lee, C.S.; Tamaru, C.S.; Miyamoto, G. T. & Kelley, C.D. 1987. Induced spawning of grey mullet (Mugil cephalus) by LHRH-a. Aquaculture 62(3): 327-336.
Levavi-Zermonsky B. & Yaron, Z. 1986. Changes in gonadotropin and ovarian steroids associated with oocytes maturation during spawning induction in the carp. General and Comparative Endocrinology 62: 89-98.
Mirhashem Rostami, S.A.; Amini, K.; Joorjani, M.; Ghezel, H.C. & Shafiee, A. 2006. An investigation on artificial reproduction of Mugil cephalus. Iranian Scientific Fisheries Journal 14 (4): 181-196. (In Farsi, Abstract in English).
Monbrison, D.D.; Tzchori, I.; Holland, M.C.; Zohar, Y.; Yaron, Z. & Elizur, A. 1997. Acceleration of gonadal development and spawning induction in the Mediterranean grey mullet, (Mugil cephalus): Preliminary studies. Bamidjeh 49(4): 214-221.
Mylonas, C.C. & Zohar, Y. 2001. Use of GnRHa-delivery systems for the control of reproduction in fish. Reviews in Fish Biology and Fisheries 10(4): 463-491.
Samira, S.A.; Alaa, A. & Mona, M.M. 2008. Reproductive biology (histological & ultrastructure) and biochemical studies in ovaries of (Mugil cephalus) from Mediterranean water. Journal of Arabian Aquaculture Society 3(1): 1- 26.
Tamaru, C.S.; Kelley, C.D.; Lee, C.S.; Aida, K., & Hanyu, I. 1989. Effects of chronic LHRH-a + 17-methyltestosterone or LHRHa + testosterone therapy on oocyte growth in the striped mullet Mugil cephalus). General and comparative endocrinology 76(1): 114-127.
Tamaru, C.S.; Lee, C.S.; Kelley, C.D.; Miyamoto, G. & Moriwake, A. 1994. Oocyte growth in the striped mullet Mugil cephalus L. maturing at different salinities. Journal of the World Aquaculture Society 25(1): 109-115.
Vazirzadeh, A.; Hajimoradloo, A.; Esmaeili, H. R.; & Akhlaghi, M. 2008. Effects of emulsified versus saline administration of GnRHa on induction of ovulation in rainbow trout, Oncorhynchus mykiss. Aquaculture 280(1): 267-269.
Vazirzadeh, A.; Mojazi Amiri, B.; Yelghi, S.; Hajimoradloo, A.; Nematollahi, M.A. & Mylonas, C.C. 2011. Comparison of the effects of different methods of mammalian and salmon GnRHa administration on spawning performance in wild-caught female carp (Cyprinus carpio carpio) from the Caspian Sea. Aquaculture 320(1): 123-128.
Yousif, O.M.; Fatah, A.A.; Krishna, K.; Minh, D.V. & Hung, B.V. 2010. Induced spawning and larviculture of grey mullet, Mugil cephalus (Linnaeus 1758) in the Emirate of Abu Dhabi. Aquaculture Asia 15(1): 1-3.
Zohar, Y. & Mylonas, C.C. 2001. Endocrine manipulations of spawning in cultured fish: from hormones to genes. Aquaculture 197(1): 99-136.