@article{JABER_ABBAS_2021, title={Molecular detection of the virulence gene’s VacA and CagA of Helicobacter pylori by PCR}, volume={8}, url={https://ijichthyol.org/index.php/iji/article/view/010045}, DOI={10.22034/iji.v8i0.708}, abstractNote={<p><em>Helicobacter pylori</em> has several virulence factors, including the ability to produce urease, which allows it to colonize the stomach and survive for long periods of time. As a results of those virulence factors of <em>H. Pylor</em>i causes gastritis. This study aimed to detect <em>H. pylori</em> using of real-time PCR amplification of <em>VacA</em> and <em>CagA</em> genes, and compare between both genes and detection by urease and creatine. For this purpose, a total of 50 patients were selected and have been clinically diagnosed with gastritis. Biopsy samples were collected from all the patients. Rapid urease test (RUT) was applied to all gastric samples to detect the presence of <em>H. Pylori</em>. DNA was extracted and the RT-PCR was perform to detect the <em>VacA</em> and <em>CagA</em> genes. The results of RUT showed 20 samples were positive of <em>H. Pylori</em>, after the Real Time-PCR test were done the results showed 27 (54%) to <em>VacA</em> and 25 (50%) to <em>CagA</em> genes were positives.</p>}, journal={Iranian Journal of Ichthyology}, author={JABER, Amany Shakeir and ABBAS, Faten Naeem}, year={2021}, month={Nov.}, pages={341–347} }