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This study was conducted to explore the Zinc oxide on the glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity of the Awassi ram sperm. The semen was divided into four groups, including control by adding Tris extender, T1= Tris extender + 0.01mg Zinc oxide/ml, T2= Tris extender + 0.04 mg\ml Zinc oxide/ml, and T3 = Tris extender + 0.08 mg\ml Zinc oxide/ ml. The diluent semen kept at 5°C. GPx and SOD were measured after reaching to 5°C (Zero time) and 72 hr. The results showed a significant (P<0.05) enhancement of GPx activity at 5°C in the T1 and T2 treatments. There were no significant changes in the T3 and the control group. After 72 hr, the T1 showed the highest GPx activity compared with the control group, whereas, no significant variation between the control and T3 groups. The SOD activity improved significantly (P<0.05) in the T1 and T2 groups as a comparison with the control group. In addition, no significant changes were found among the control and T3 groups at 5 and 72 hr of cooling.


Awassi ram Zinc oxide GPx SOD

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NEAMAH, H. J. ., AL-OMAIRI, S. G. F., & AL-SARRAY, M. A. R. (2022). The effect of zinc oxide on the glutathione peroxidase and superoxide dismutase enzymes activities of ram sperm at cooling. Iranian Journal of Ichthyology, 9, 462–466. Retrieved from


  1. Adewoyin, M.; Ibrahim M.; Roszaman, R.; Isa, M.; Alewi, N.; Rafa, A. & Anuar, M. 2017. Male Infertility: The Effect of Natural Antioxidants and Phytocompounds on Seminal Oxidative Stress. Diseases (Basel, Switzerland) 5(1): 9.
  2. Afifi, M.; Almaghrabi, O.A. & Kadasa, N.M. 2015. Ameliorative Effect of Zinc Oxide Nanoparticles on Antioxidants and Sperm Characteristics in Streptozotocin-Induced Diabetic Rat Testes. BioMed Research International 153573.
  3. Ahuja, A. & Parmar, D. 2017. Role of minerals in reproductive health of dairy cattle: a review. International Journal of Livestock Research 7(10): 16-26.
  4. Alavi-Shoushtari, S.M.; Rezai, S.A.; Ansari, M.H.K. & Khaki, A. 2009. Effects of the seminal plasma zinc content and catalase activity on the semen quality of water buffalo (Bubalus bubalis) bulls. Pakistan Journal of Biological Sciences 12(2): 134-139.
  5. AL-Sarray, M.A.R. 2019. Effect of gelatin, lycopene, selenium nanoparticles and their combinations added to Tris semen on turkey Awassi rams semen quality under cooling and freezing condition. Ph.D. Thesis. Agricultural Engineering Sciences, University of Baghdad.
  6. Al-Sarray, M.A.R.; Hobi, A.A. & Al-Ani, A.A.ET. 2019. Effect of adding lycopene to the Tris extender on Awassi rams semen quality preseveded at cooling and freezing storage. Plant Archives 19(2): 3971-3976.
  7. Al-Sarry, M.A.R.; Hobi, A.A. & Al-Ani, A.A.E.T. 2020. The effect of adding of selenium nanoparticles to the tris extender on Awassi rams semen quality persevered at cooling and freezing storage. Biochemical and Cellular Archives 20(1): 543-548.
  8. Al-Subaihawi, H.R.B.; Al-Saab, H.K.J. & Al-Sarray, M.A. 2017. Extrication and lyophilization low-density lipoproteins and used it instead of egg yolk in semen extender of awassi rams and their influences in sperm abnormalities store at 5°C. The Iraqi Journal of Agricultural Sciences 48(1): 342-349.
  9. Banana, H.J.H.; Neamah, H.J. & Saieed, A.Y. 2020. Evaluation of adding aqueous extract of Opuntia Ficus Indica leaves to Tris extender on the criteria of Awassi rams semen stored at cooling. Indian Journal of Forensic Medicine & Toxicology 15(3).
  10. Bansal, A.K. & Bilaspuri, G.S. 2010. Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International 686137.
  11. Beauchamp, C. & Fridovich, I. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44 (1): 276-287.
  12. Bettger, W. & O’Dell, BA. 1981. Critical physiological role of zinc in the structure and function of biomembranes. Life Sciences 28(13): 1425-1438:
  13. Briggs, O.N.; Brown, H.; Elechiamadi, K.; Ezeiruaku, F. & Nduka, N. 2016. Superoxide dismutase and glutathione peroxidase levels in patients with long standing type2 diabetes in Port Harcourt, Rivers State, Nigeria. International Journal Sciences Research 5(40).
  14. Colagar, A.H.; Marzony, E.T.; Chaichi, M.J. 2009. Zinc levels in seminal plasma are associated with sperm quality in fertile and infertile men. Nutrition Research 29(2): 82-88:
  15. Daghigh-Kia, H.; Olfati-Karaji, R.; Hoseinkhani, A. & Ashrafi, I. 2014. Effect of rosemary (Rosmarinus officinalis) extracts and glutathione antioxidants on bull semen quality after cryopreservation. Spanish Journal of Agricultural Research 12(1): 98-10.
  16. Dani, V. & Dhawan, K. 2011. Radioprotective role of zinc following single dose radioiodine (131I) exposure to red blood cells of rats. Journal of Environmental Pathology, Toxicology and Oncology 30(2): 153-162.
  17. Eggert Kruss, W.; Zwick, E.M.; Batschulat, K.; Rohr, G.; Armbruster, F.P.; Petzoldt, D. & Strowitzki, T. 2002. Are zinc level in seminal plasma associated with seminal leukocyte and other determinant of semen quality? Fertility and Sterility 77(2): 260-269:
  18. Eidan, S.M. 2016. Effect on post-cryopreserved semen characteristics of Holstein bulls of adding combinations of vitamin C and either catalase or reduced glutathione to Tris extender. Animal Reproduction Science 167: 1-7:
  19. Evans, G. & Maxwell, W. 1987. Salamon’s artificial insemination of sheep and goats. Butterworth, Sydney, 194 p.
  20. Gabr, A.A. & Basuini, M.F. 2018. Effect of tonophosphan, zinc oxide, and ascorbic acid on semen, sexual desire, and the fertility rate of Egyptian buffalo bulls. Annals of Agricultural Sciences 63(2): 215-221:
  21. Gebreselassie, G.A.; Srivastava, S.K.; Ghosh, S.K. & Tripathi, R.P. 2013. Level of extra and intracellular enzymes during preservation of good and poor quality ejaculates in crossbred bulls. Indian Journal of Animal Sciences 83(7): 697-699.
  22. Halo, M.J.; Bułka, K.; Antos, P.A.; Greń, A.; Slanina, T.; Ondruška, Ľ.; Tokárová, K.; Massányi, M.; Formicki, G.; Halo, M. & Massányi, P. The effect of ZnO nanoparticles on rabbit spermatozoa motility and viability parameters in vitro. Saudi Journal of Biological Sciences 28(12): 7450-7454.
  23. dos Santos Hamilton, T.R.; de Castro, L.S.; de Carvalho Delgado, J.; de Assis, P.M.; Siqueira, A.F.P.; Mendes, C.M. & Assumpção, M.E.O.D.Á. 2016. Induced lipid peroxidation in ram sperm: semen profile, DNA fragmentation and antioxidant status. Reproduction, 151(4), 379-390.
  24. Hazarika, S.B.; Bhuyan, D; Deka, B.C.; Sinha, S.; Biswas, R.K.; Dutta, D.J.; Das, A.; Borah, P. & Dewry, R. K. 2016. Effect of glutathione on the quality of frozen buck semen. Indian Journal of Animal Sciences 86(5): 535-538.
  25. Heidari, J.; Jamal Seifdavati, J.; Mohebeddini, H.; Seyed Sharifi, R. & Abdi Benemar, H. 2019. Effect of nano zinc oxide on post-thaw variables and oxidative status of Moghani ram semen. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 25(1): 71-76.
  26. Hollis, G.R.; Carter, S.D.; Cline, T.R.; Crenshaw, T.D.; Cromwell, G.L.; Hill, G.M.; Kim, S.W.; Lewis, A.J.; Mahan, D.C.; Miller, P.S.; Stein, H.H. & Veum, T.L. 2005. Effects of replacing pharmacological levels of dietary zinc oxide with lower dietary levels of various organic zinc sources for weanling pigs. Journal of Animal Sciences 83(9): 2123-2129.
  27. Makker, K.; Agarwal, A. & Sharma, R. 2009. Oxidative stress and male infertility. Indian Journal of Medical Research, 129(4): 357-367.
  28. Mortazavi, M.; Salehi, I.; Alizadeh Z.; Vahabian, M. & Roushandeh, A.M. 2014. Protective Effects of Antioxidants on Sperm Parameters and Seminiferous Tubules Epithelium in High Fat-fed Rats. Journal of Reproduction and Infertility 15(1):22-28.
  29. Nagamine, C.M.; Chan, K.; Hake, L.E. & Lau, Y.F.C. 1990. The two-candidate testis determining Y genes (Zfy-1 and Zfy-2) are differentially expressed in fetal and adult mouse tissues. Genes and Development 4(1): 63-74.
  30. Neamah, H.J. & Houbi, AA. 2020. Effect of adding astaxanthin and taurine to the Tris extender on the semen quality of Awassi rams during cooling storage. Annals of Tropical Medicine and Public Health 23(7):
  31. Ozsoy, N.; Can, A.; Mutlu, O.; Akev, N. & Yanardag, R. 2012. Oral zinc supplementation protects rat kidney tissue from oxidative stress in diabetic rats. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 8(4): 545-550.
  32. Paál, D.; Strejček, F.; Tvrdá, E.; Formicki, G.; Klein, S.; Rath, De. & Massanyi, P. 2018. The in vitro effect of taurine on boar spermatozoa quality. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 66:131-137.
  33. Powell, S R. 2000.The antioxidant properties of zinc. The Journal of Nutrition 130(5): 1447-1454.
  34. Sabhapati, M.; Raina, V.S.; Bhakat, M.; Mohanty, T.K.; Shivahre, P.R.; Mondal, G. & Gupta, A.K. 2016. Improvement of sexual behavior and semen quality by therapeutic approach and zinc supplementation on Karan Fries. Indian Journal of Animal Science 86 (6): 655-658.
  35. Tran, L.V.; Malla B.A.; Kumar S. & Tyagi A.K. 2017. Polyunsaturated fatty acids in male ruminant reproduction-A review. Asian-Aust. Journal of Animal Science 30 (5): 622–637:
  36. Zhang, X.G.; Yan, G.J.; Hong, J.Y.; Su, Z.Z.; Yang, G.S.; Li, Q.W. & Hu, J.H. 2015. Effects of bovine serum albumin on boar sperm quality during liquid storage at 17°C. Reproduction in Domestic Animals 50: 263-269.