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Abstract

The present work aimed to collect various types of mushrooms from different regions in Basrah province and their identification based on morphological and molecular data using ITS1-ITS4 primers. During the present study, eight taxa were identified viz. wild Agaricus bisporus, A. bitorquis, Coprinopsis picaceus, Panaeolus campanulatus, P. papilionaceus, Psathyrella candolleanl, Psathyrella sp.1 and Psathyrella sp.2. Based on sequencing results, five new strains were recorded in the gene bank: two strains of A. bisporus (A. bisporus IQ Inaam1 and A. bisporus IQ Nassar), two strains of P. papilionaceus (P. papilionaceus INNASA2 and P. papilionaceus IQ Zeena), and one strain of P. candolleana as a new globally (P. candolleana IQ M.Jawad).

Keywords

Mushrooms Molecular Morphological identification New strains

Article Details

How to Cite
ALRUBAYAE, I. M., MAKTOOF, S. A., & AL MANSOUR, N. A. (2022). Molecular and morphological identification of wild mushrooms isolated in Basrah Province with the global recording of new strains. Iranian Journal of Ichthyology, 9, 320–333. Retrieved from https://ijichthyol.org/index.php/iji/article/view/818

References

    Al-Khesraji, T.O.; Shugran, A.H. & Augul, R.S. 2017. Some Basidiomycota Macrofungal Species from Salahadin Governorate (North Central Iraq), with the Addition of Four New Species to Iraq. International Journal of Current Research in Biosciences and Plant Biology 4(10): 74-84.
    Appiah, T.; Agyare, C. & Luo, Y. 2017. Molecular identification of some ghanaian mushrooms using internal transcribed spacer regions. Molecular Biology 6: 3.
    Aziz, F.H. & Toma, F.M. 2012. First observations on the mushroom in Mountain Area of Iraqi Kurdistan Region. J. Advanced Laboratory Research in Biology 3: 4.
    Bazzle, L.; Cubeta, M.; Marks, S. & Dorman, D. 2015. Feasibility of flotation concentration of fungal spores as a method to identify toxigenic Mushrooms. Veterinary Medicine: Research and Reports 6: 1-9.
    Chang, S.T. & Miles, P.G. 2004. Mushrooms Cultivation, Nutritional Value, Medicinal Effect and Environmental Impact. 2nd End, CRC Press, Boca Raton. 450 p.
    Dung, N.T.P.; Tuyen, D.B. & Quang, P.H. 2012. Morphological and genetic characteristics of oyster mushrooms and conditions affecting on its spawn growing. International Food Research Journal 19(1): 347-352.
    Froslev, T.; Jeppesen, T.; Laessoe, T. & Kjoller, R. 2007. Molecular phylogenetics and delimitation of species in Cortinarius section Calochroi (Basidiomycota, Agaricales) in Europe. Molecular Phylogenetics and EVOLUTION. 44: 217-27.
    Hall, I.R.; Stephenson, S.L.; Buchanan, P.K.; Yun, W. & Cole, A.L.J. 2003. Edible and Poisonous Mushrooms of the World. Printed through Colorcraft Ltd., Hong Kong. 370 p.
    Hibbett, D.S.; Binder, M.; Bischoff, J.F.; Blackwell, M.;Cannon, P.F.; Eriksson, O.E.; Huhndorf, S.; James, T.; Kirk, P.M.; Lucking, R.; Lumbsch, H.T.; Lutzoni, F.; Matheny, P.B.; Mclaughlin, D.J.; Powell, M.J.; Redhead, S.; Schoch, C.L.; Spatafora, J.W.; Stalpers, J.A.; Vilgalys, R.; Aime, M.C.; Aptroot, A.; Bauer, R.; Begerow, D.; Benny, G.L.; Castlebury, L.A.; Crous, P.W.; Dai, Y.C.; Gams, W.; Geiser, D.M.; Griffith, G.W.; Gueidan, C.; Hawksworth, D.L.; Hestmark, G.; Hosaka, K.; Humber, R.A.; Hyde, K.D.; AIronside, J.E.; Koljalg, U.; Kurtzman, C.P.; Larsson, K.H.; Lichtwardt, R.; Longcore, J.; Miadlikowska, J.; Miller, A.; Moncalvo, J.M.; Mozley-Standridge, S.; Oberwinkler, F.; Parmasto, E.; Reeb, V.; Rogers, J.D.; Roux, C.; Ryvarden, L.; Sampaio, J.P.; Schussler, A.; Sugiyama, J.; Thorn, R.G.; Tibell, L.; Untereiner, W.A.; Walker, C.; Wang, Z.; Weir, A.; Weiss, M.; White, M.M.; Winka, K.; Yao, Y.J. & Zhang, N. 2007. A higher-level phylogenetic classification of the fungi. Mycological Research 111: 509-547.
    Khalaf, K.T. 2015. Isolation and Identification of two bioactive compounds from basidiomycete’s fungus Coprinus sp. Journal of Thi-Qar Sciences 5(2): 11.
    Lee, J.S.; Lim, M.O.; Cho, K.Y.; Cho, J.H.; Chang, S.Y. & Nam, D.H. 2006. Identification of medicinal mushroom species based on nuclear large subunit rDNA sequences. Journal of Microbiology 44(1): 29-34.
    Leon, A.M.; Luangsa-ard, J.; Karunarathna, S.C. Hyde, K.D.; Reyes, RG. & Cruz T. 2013. Species listing, distribution, and molecular identification of macrofungi in six Aeta tribal communities in Central Luzon,, Philippines. Mycosphere 4(3): 478-494.
    Manjunathan, J.; Kumar, M. & Kaviyarasan, V. 2011. Taxonomic studies, rapid and efficient protocol for DNA extraction, purification, and molecular characterstics of the basidiomycete Lentinus tuberregium (FR) GQ292711. Asian Journal of Pharmaceutical and Clinical Research 4: 54-58.
    Mirhendi, H.; Makimura, K.; Khoramizadeh, M. & Yamagushi, H. 2006. A one-enzyme PCR-RFLP assay for identification of six medically important Candida species. Japanese Journal of Microbiology 47: 225-229.
    Muhsin, T.; Abass, A. & Al-Habeeb, E. 2012. Podaxis pistillaris (Gasteromycetes) from the desert of southern Iraq, an addition to the known mycota of Iraq. Journal of Basrah Researches (Sciences) 38(3).
    Raja, H.A.; Baker, T. R.; Little, J.G. & Oberlies, N.H. 2017b. DNA barcoding for identification of consumer-relevant mushrooms: A partial solution for product certification. Journal of Food Chemistry 214: 383-392.
    Raja, H.A.; Miller, A.N.; Pearce, C.J. & Oberlies, N.H. 2017a. Fungal Identification Using Molecular Tools: A primer for the natural products research community. Journal of Natural Product 80: 756-770.
    Rajaratnam, S. & Thiagarajan, T. 2012. Molecular characterization of wild mushroom. European Journal of Experimental Biology 2(2): 369-373.
    Rajesh, K.; Dhanasekaran, D. and Panneerselvam, A. 2014. Isolation and taxonomic characterization of medicinal mushroom Ganoderma spp. Academia Journal of Microbiology Research 2(2): 61-70.
    Sanchez-Ballesteros, J.; Gonzalez, V.; Salazar, O.; Acero, J.; Portal, M.A.; Julia´n, M.; Rubio, V.; Bills, G.F.; Polishook, J.D.; Platas, G.; Mochales, S. & Pelaez, F. 2000. Phylogenetic study of Hypoxylon and related genera based on ribosomal ITS sequences. Mycologia 92: 964-977.
    Suliaman, S.Q.; Khesraji, T.O. & AL- Hassan, A.A. 2017. New records of basidiomycetous macrofugi from Kurdistan region-Northern Iraq. African Journal of Plant Science 11(6): 209-219.
    Tiwari, P.; Nathiya, R. & Mahalingam, G. 2017. Antidiabetic activity of endophytic fungal isolated from Ficus religiosa. Pharmaceutical and Clinical Research 10: 59-61.
    Toma, F.; Ismael, N. & Abdulla F. 2018. Survey and Identification of some New Record Mushrooms in Erbil Governorate-Kurdistan Region-Iraq. Special Issue for the Third Scientific Conference of Biology 5: 19-32.
    Urbanelli, S.; Della Rosa, V.; Punelli, F.; Porretta, D.; Reverberi, M.; Fabbri, AA. & Fanelli, C. 2007. DNA-fingerprinting (AFLP and RFLP) for genotypic identification in species of the Pleurotus eryngii complex. Applied Microbiology and Biotechnology 74: 592-600.