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Abstract
This study aimed to analysis the composition of Artemisia herba-alba flowers’ essential oils using GC-MS and its antioxidant properties. The inhibitory activity was determined using five different concentrations of essential oil of A. herba-alba flowers extracts. The results showed that the antioxidant activity of total essential oils of A. herba-alba flowers is 97.8% in 20μl/ml, while the lower inhibition was 36.1% in 2μl/ml compared to the standard vitamin E. The lowest inhibitory activity was at a concentration of 2μl/ml if the percentage reached 8.5%. The highest antioxidant activity was recorded at a concentration of 20μl/ml as 93.6%. The inhibition percentage of A. herba-alba found to be 97.8% in 20μg/ml. Total antioxidant capacity of A. herba-alba was y = 4.2592x+15.87, R²=0.912. Artemisia herba-alba had compounds 61 components in its essential oil such as 2-Cyclohexen-1-one, 3-methyl-6-(1-methylethyl) (22.24%), 2-Cyclohexen-1-one, 3-methyl-6-(1-methylethyl) (13.99%), (2S,6R,7S,8E)-(+)-2,7-Epoxy-4,8-megastigmadiene (9.48%), Bicyclo[2.2.1]heptane-2,3-dione, 1,7,7-trimethyl-, (1S) (8.33%), Carbamic acid, monoammonium (8.58%), Isophorone (5.22%) and Camphor (4.66%).
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The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
References
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Paolini, J.; Ouariachi, E.; Bouyanzer, A.; Hammouti, B.; Desjobert, J.M.;, Costa, J. & Muselli, A. 2010. Chemical variability of Artemisia herba-alba Asso essential oils from East Morocco. Chemical Papers 64(5): 550-556.
Shahidi, F. 2008. Antioxidants: Extraction, identification, application, and efficacy measurement. Electronic Journal of Environmental, Agricultural and Food Chemistry 7(8): 3325-3330.
Tahraoui, A.; El-Hilaly, J.; Israili, Z.H. & Lyoussi, B. 2007. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in south-eastern Morocco (Errachidia province). Journal of Ethnopharmacology 110: 105-117.
Vernin, G.; Merad, O.; Vernin, G.M.F.; Zamkotsian, R.M. & Párkányi, C. 1995. GC-MS analysis of Artemisia herba alba Asso essential oils from Algeria. In: G. Charalambous (Ed.), Food flavors: Generation, analysis and process influence. Amsterdam, the Netherlands: Elsevier. pp: 147-205.
References
Aloui, Z.; Meriam, C.H.; Neffati, N.; Jamoussi, I.B.; Essafi-Benkhadir, K.; Boussaid, M.; Guizani, I. & Karoui, H. 2016. Asteraceae Artemisia campestris and Artemisia herba-alba essential oils trigger apoptosis and cell cycle arrest in Leishmania infantum promastigotes, Evidence-Based Complementary and Alternative Medicine 1-16.
Assellaou A. 213. Master dissertation. Ecole Nationale Supérieure Agronomique de Montpellier, 2013.
Blois, M.S. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181 (4617): 1199-1200.
Daycem, K.; Rabiaa, M.S. Sameh, A.; Dhafer, Laouinicd, M. Hamdi. & Jalloul, B. 2013. Composition and anti-oxidant, anti-cancer and anti-inflammatory activities of Artemisia herba-alba, Ruta chalpensis L. and Peganum harmala L. Food and Chemical Toxicology 55: 202-208.
Dob, T. & Benabdelkader, T. 2006. Chemical Composition of the Essential Oil of Artemisia herba-alba Asso Grown in Algeria. Journal of Essential Oil Research 18: 685-690.
Feuerstein, I.; Barel, S. & Segal, R. 1987. The antibacte- rial and antispasmodic activity of Artemisia herba-alba Asso. II. Examination of essential oils from various chemotypes. International Journal of Crude Drug Research 25: 89-96.
Hatano, T.; Kagawa, H.; Yasuhara, T. & Okuda, T. 1988. Two new flavonoids and other constituents in Licorice Root: Their relative astringency and radical scavenging effects. Chemical and Pharmaceutical Bulletin 36: 2090-2097.
Hudaib, M.M. & Aburjai, T.A. 2006. Composition of the essential oil from Artemisia herba-alba grown in Jordan. Journal of Essential Oil Research 18(3): 301-304.
Imeri, A.; Kupe, L.; Shehu, J.; Dodona, E.; Bardhi, N. & Vladi, V. 2014. Essential oil composition in three cultivars of Ocimum L. in Albania. Archives of Biological Sciences 66(4): 1641-1644.
Joshi, R.K. 2014. Chemical composition and antimicrobial activity of the essential oil of Ocimum basilicum L.(sweet basil) from Western Ghats of North West Karnataka, India. Ancient Science of Life 33(3): 151-156.
Khelifa, L.H.; Brada, M.; Brahmi, F.; Achour, D.; Fauconnier, M.L. & Lognay, G. 2012. Chemical composition and antioxidant activity of essential oil of Ocimum basilicum leaves from the northern region of Algeria. Topclass Journal of Herbal Medicine 1(2): 53-58.
Makhlouf M.H. & Layka S. 2011. Study the Biodiversity of the Asteraceae Family in Lattakia Province. Syria. Journal of the University of Damascus Basic Sciences 2: 300-314
Marrif, H.I.; Ali, B.H. & Hassan, K.M. 1995. Some pharmacological studies on Artemisia herba-alba (Asso) in rabbits and mice. Journal of Ethnopharmacology 49: 51-55.
Merghem, R.; Jay, N.; Burn, N. & Voirin, B. 2004. Quantitative analysis and HPLC isolation and identification of procyanidins from V'icia faba L. Phytochemistry Analysis 15: 95-99.
Mosquera, O.M.; Correa, Y.M.; Buitrago, D.C. & Nino, J. 2007. Antioxidant activity of twenty five plants from Colombian biodiversity. Memorias Instituto Oswaldo Cruz 102: 631-634.
Al-Wahaibi, L.H.N.; Mahmood, A.; Khan, M. & Alkhathlan H. Z. 2020. Comparative study on the essential oils of Artemisia judaica and A. herba-alba from Saudi Arabia. Arabian Journal of Chemistry 13(1): 2053-2065.
Paolini, J.; Ouariachi, E.; Bouyanzer, A.; Hammouti, B.; Desjobert, J.M.;, Costa, J. & Muselli, A. 2010. Chemical variability of Artemisia herba-alba Asso essential oils from East Morocco. Chemical Papers 64(5): 550-556.
Shahidi, F. 2008. Antioxidants: Extraction, identification, application, and efficacy measurement. Electronic Journal of Environmental, Agricultural and Food Chemistry 7(8): 3325-3330.
Tahraoui, A.; El-Hilaly, J.; Israili, Z.H. & Lyoussi, B. 2007. Ethnopharmacological survey of plants used in the traditional treatment of hypertension and diabetes in south-eastern Morocco (Errachidia province). Journal of Ethnopharmacology 110: 105-117.
Vernin, G.; Merad, O.; Vernin, G.M.F.; Zamkotsian, R.M. & Párkányi, C. 1995. GC-MS analysis of Artemisia herba alba Asso essential oils from Algeria. In: G. Charalambous (Ed.), Food flavors: Generation, analysis and process influence. Amsterdam, the Netherlands: Elsevier. pp: 147-205.