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Abstract

Due to an increased resistance to antifungal medicines, the prevalence of hospital-acquired fungal infection has recently increased. This study aimed to determine the virulence features that assist in increasing the pathogenicity of clinical yeast isolates. For this purpose, 61 clinical yeast isolates were ued to examine their enzymatic activity (proteinase, phospholipase, and hemolysin), potential to form biofilms, and antifungal susceptibility patterns. The results indicated that majority of the yeast isolates exhibited potential proteinase activity, with 24.6% exhibiting weak activity, 19.7% moderate activity, and 9.8% high activity, while 45.9% phospholipase activity, with 16.4% weak activity, 24.6% exhibiting moderate activity, and 4.9% strong activity. While hemolysin production was demonstrated in 85.2%, that 59.0% were strong, 21.3% moderate, and 4.9% weak. Additionally, it was possible to identify biofilm development, which occurred in 90.2% of isolates. All isolates showed sensitivity to the antifungals tested, with the exception of one Candida glabrata isolate that demonstrated resistance to voriconazol and two Candida parapsilosis isolates resistance to flucytosine. The results also revealed no significant changes in proteinase activity or drug susceptibility profile, but significant variations in phospholipase, hemolysin, and biofilm generation amongst yeast isolates.

Keywords

Nosocomial infections Candida Enzymatic activity Biofilm Antifungal susceptibility test

Article Details

How to Cite
ABEED, F. K., & ALRUBAYAE, I. M. (2022). Evaluation of virulence factors of clinical yeast isolates from nosocomial fungal infections with the determination of their antifungal susceptibility profile. Iranian Journal of Ichthyology, 9, 61–68. Retrieved from https://ijichthyol.org/index.php/iji/article/view/764

References

    Al-laaeiby, A.I.E.; Al-Mousawi, A.A.; Alrubayae, I.; Al-Saadoon, A. & Almayahi, M. 2020. Innate pathogenic traits in oral yeasts. Karbala International Journal of Modern Science 6(4): 5.
    AL-Rubayae, I.M. 2013. Isolation and Identification of Candida species Associated with Urinary Tract Infections. Department of biology, Collage of Science, University of Basra.
    Al-Rubayae, I.M.; Al-laaeiby, A.; Minati, M.H. & ALibraheem, S.A. 2020. Determination of genetic relationships and pathogenicity of oral candidiasis etiological agents in pediatric malignant patients in Basrah province, Iraq. Systematic Reviews in Pharmacy 11.
    Aoki, S.; Ito-Kuwa, S.; Nakamura, Y. & Masuhara, T. 1990. Comparative pathogenicity of a wild-type strain and respiratory mutants of Candida albicans in mice. Zentralblatt für Bakteriologie 273(3): 332-343.
    deMelo, A.V.; Zuza-Alves, D.L.; da Silva-Rocha, W.P.; de Souza, L.F.C.; Francisco, E.C.; de Azevedo Melo, A.S. & Chaves, G.M. 2019. Virulence factors of Candida spp. obtained from blood cultures of patients with candidemia attended at tertiary hospitals in Northeast Brazil. Journal De Mycologie Medicale 29(2): 132-139.
    El-Kholy, M.A.; Helaly, G.F.; El Ghazzawi, E.F.; El-Sawaf, G. & Shawky, S.M. 2021. Virulence Factors and Antifungal Susceptibility Profile of C. tropicalis Isolated from Various Clinical Specimens in Alexandria, Egypt. Journal of Fungi 7(5): 351.
    Freeman, D.J.; Falkiner, F.R. & Keane, C.T. 1989. New method for detecting slime production by coagulase negative staphylococci. Journal of Clinical Pathology 42(8): 872-874.
    Fridkin, S.K. & Jarvis, W.R. 1996. Epidemiology of nosocomial fungal infections. Clinical Microbiology Reviews 9(4): 499-511.
    Hasan, F.; Xess, I.; Wang, X.; Jain, N. & Fries, B.C. 2009. Biofilm formation in clinical Candida isolates and its association with virulence. Microbes and Infection 11(8-9): 753-761.
    Jahagirdar, V.L.; Davane, M.S.; Aradhye, S.C. & Nagoba, B.S. 2018. Candida species as potential nosocomial pathogens--A review. Electronic Journal of General Medicine 15(2): 1.
    Joshua, P.; Bryan, C. & Brad, S. 2006. Nosocomial fungal infections: epidemiology, diagnosis, and treatment. Medical Mycology 45: 321-346.
    Luo, G.; Samaranayake, L.P. & Yau, J.Y. 2001. Candida species exhibit differential in vitro hemolytic activities. Journal of Clinical Microbiology 39(8): 2971-2974.
    Othman, K.I.; Shoris, M.A.; Ali, B. & Mjda, M. 2018. Isolation and identification Candida spp. from urine and antifungal susceptibility test. Biology department, Faculty of Science, Soran University, Soran- Erbil, Iraq.
    Price, M.F.; Wilkinson, I.D. & Gentry, L.O. 1982. Plate method for detection of phospholipase activity in Candida albicans. Sabouraudia 20: 7-14.
    Rossoni, R.D.; Barbosa, J.O.; Vilela, S.F.G.; Jorge, A.O.C. & Junqueira, J.C. 2013. Comparison of the hemolytic activity between C. albicans and non-albicans Candida species. Brazilian Oral Research 27: 484-489.
    Samaranayake, L.P.; Raeside, J.M. & MacFarlane, T.W. 1984. Factors affecting the phospholipase activity of Candida species in vitro. Sabouraudia: Journal of Medical and Veterinary Mycology 22(3): 201-207.
    Sida, H.; Shah, P.; Pethani, J.; Patel, L. & Shah, H. 2016. Study of biofilm formation as a virulence marker in Candida species isolated from various clinical specimens. International Journal of Medical Science and Public Health 5(5): 842-846.
    Sriphannam, C.; Nuanmuang, N.; Saengsawang, K.; Amornthipayawong, D. & Kummasook, A. 2019. Anti-fungal susceptibility and virulence factors of Candida spp. isolated from blood cultures. Journal De Mycologie Medicale 29(4): 325-330.
    Tellapragada, C.; Eshwara, V.K.; Johar, R.; Shaw, T.; Malik, N.; Bhat, P.V. & Mukhopadhyay, C. 2014. Antifungal susceptibility patterns, in vitro production of virulence factors, and evaluation of diagnostic modalities for the speciation of pathogenic Candida from bloodstream infections and vulvovaginal candidiasis. Journal of Pathogens 2014.
    Vijayalakshmi, P.; Thenmozhi, S. & Rajeswari, P. 2016. The Evaluation of the virulence factors of clinical Candida isolates and the anti-biofilm activity of Elettaria cardamomum against multi-drug resistant Candida albicans. Current Medical Mycology 2(2): 8.