Main Article Content
Abstract
The resistance against disease is granted by one incompletely dominant gene existing on chromosome eleventh. Gray leaf spot is caused by Stemphylium sp.. This study aimed to distinguish the cultivar resistant alleles or susceptible alleles by molecular marker linked to Resistance Gene (Sm gene) using Marker Assisted Selection (MAS) inbreeding. In this study, we used eight tomato cultivars farmed in Iraq. The results showed that the codominant marker InDe, after PCR amplification, produced a 122bp fragment for resistance in seven genotypes and a 140bp fragment of susceptible alleles in one genotype, respectively could be utilized in MAS for resistance disease of gray leaf spot.
Keywords
Gray leaf spot
Sm gene
Tomato
Indel markers.
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The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
How to Cite
ALRUFAYE, Z. T. A., GHALEB, I. K., ABIDUKADHIM, A. R., & Safaa, Z. (2022). Detection of resistance gene to gray leaf spot caused by Stemphylium spp. in tomato cultivars of Iraq. Iranian Journal of Ichthyology, 9, 54–56. Retrieved from https://ijichthyol.org/index.php/iji/article/view/762
References
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Behare, J.; Laterrot, H.; Sarfatti, M. & Zamir, D. 1991. Restriction fragment length polymorphism mapping of the Stemphylium resistance gene in tomato. Molecular Plant-Microbe Interactions 4: 489-492.
Blancard, D.; Laterrot, H.; Moretti, A.; Pichot, M. 1986. Les Stemphylia rencontrés sur tomate. Phytopathologia Mediterranea 25: 140-144.
Chai, A.L.; Du, G.F.; Shi, Y.X.; Xie, X.W. & Li, B.J. 2015. Leaf spot on sweet potato (Ipomoea batatas) caused by Stemphylium solani, a new disease in China. Journal of Phytopathology 163: 1046-1049.
Cho, H.J.; Kim, B.S. & Hwang, H.S. 2001. Resistance to gray leaf spot in capsicum peppers. Hortscience 36(4): 752-754.
Hendrix, J.W. & Frazier, W.A. 1949. Studies on the inheritance of Stemphylium resistance in tomatoes. Hawaii Agricultural Experiment Station, Technical Bulletin 8.
Kim, B.S.; Cho, H.J.; Hwang, H.S. & Cha, Y.S. 1999. Gray leaf spot of tomato caused by Stephylium solani. The Plant Pathology Journal 15: 348-350.
Krattinger, S.G. 2009. Molecular characterization of the durable broadspectrum disease resistance gene Lr34 in wheat (Triticum aestivum L.). Universität Zürich, Zürich.
Li, B.; Zhou, Y.; Li, J. & Xie, X. 2010. Diagnosis of diseases by Dr Li Bao Ju: the diagnosis and prevention of tomato Stemphylium leaf spot disease (gray leaf spot). China Veg 24-25.
Lin, T.; Zhu, G.; Zhang, J.; Xu, X.; Yu, Q.; Zheng, Z.; Zhang, Z.; Lun, Y.; Li, S.; Wang, X.; Huang, Z.; Li, J.; Zhang, C.; Wang, T.; Zhang, Y.; Wang, A.; Zhang, Y.; Lin, K.; Li, C.; Xiong, G.; Xue, Y.; Mazzucato, A.; Causse M.; Fei, Z.; Giovannoni, J.J.; Chetelat, R.T.; Zamir, D.; Stadler, T.; Li, J.;Ye, Z.; Du, Y. & Huang, S. 2014. Genomic analyses provide insights into the history of tomato breeding. Nature Genetics 46: 1220-1226
Miranda, B.E.C. & Bolteux, L.S. 2010. Identification REISA. Of Solanum (section lycopersicon) accessions with resistance to Stemphylium solani and S. Lycopersici. Horticultura Brasileira 28: 178-184.
Nasehi, A.; Kadir, J.B.; Esfahani, M.N.; Mahmodi, F.; Ghadirian, H.; Abed Ashtiani, F. & Golkhandan, E. 2013. An outbreak of leaf spot caused by Stemphylium solani on eggplant in Malaysia. Plant Disease 97: 689
Nasehi, A.; Kadir, J.B.; Zainal Abidin, M.A.; Wong, M.Y. & Abed Ashtiani, F. 2012a. First report of gray leaf spot on pepper caused by Stemphylium solani in Malaysia. Plant Disease 96: 1227.
Rani L.; Bai C. & Singh, R.P.2020.Genes, QTLs and linked molecular markers on ferring the resistance for various biotic stresses in tomato cultivar. Journal of Entomology and Zoology Studies 8(1):971-977.
Simmons, E.G. 2001. Perfect states of Stemphylium-IV. Harvard Papers in Botany 199-208.
Su, X.; Zhu, G.; Huang, Z.; Wang, X.; Guo, Y.; Li, B. & Gao, J. 2019. Fine mapping and molecular marker development of the Sm gene conferring resistance to gray leaf spot (Stemphylium spp.) in tomato. Theoretical and Applied Genetics 132(4): 871-882.
Thamer Abdul Hussein Al-Rufaye, Z.; Muhammed Sheriff Al-Sheikh, W. & Kadhum Ghaleb, I .2018. Detection of Lgc1 Gene Low Glutelin Content in Rice CultivarsIraq using Indel Markers. Journal of Global Pharma Technology| 10(08): 193-196.
Yang, H.; Zhao, T.; Jiang, J.; Wang, S.; Wang, A.; Li, J. & Xu, X. 2017. Mapping and screening of the tomato Stemphylium lycopersici resistance gene, Sm, based on bulked segregant analysis in combination with genome resequencing. BMC Plant Biology 17(1): 1-10.
Yang, H.H.; Xu, X.Y.; Zhao, T.T. & Li, J.F. 2017. First Report of Stemphylium lycopersici causing gray Leaf Spot on Eggplant in China. Plant Disease 101(5): 834.
References
Behare, J.; Laterrot, H.; Sarfatti, M. & Zamir, D. 1991. Restriction fragment length polymorphism mapping of the Stemphylium resistance gene in tomato. Molecular Plant-Microbe Interactions 4: 489-492.
Blancard, D.; Laterrot, H.; Moretti, A.; Pichot, M. 1986. Les Stemphylia rencontrés sur tomate. Phytopathologia Mediterranea 25: 140-144.
Chai, A.L.; Du, G.F.; Shi, Y.X.; Xie, X.W. & Li, B.J. 2015. Leaf spot on sweet potato (Ipomoea batatas) caused by Stemphylium solani, a new disease in China. Journal of Phytopathology 163: 1046-1049.
Cho, H.J.; Kim, B.S. & Hwang, H.S. 2001. Resistance to gray leaf spot in capsicum peppers. Hortscience 36(4): 752-754.
Hendrix, J.W. & Frazier, W.A. 1949. Studies on the inheritance of Stemphylium resistance in tomatoes. Hawaii Agricultural Experiment Station, Technical Bulletin 8.
Kim, B.S.; Cho, H.J.; Hwang, H.S. & Cha, Y.S. 1999. Gray leaf spot of tomato caused by Stephylium solani. The Plant Pathology Journal 15: 348-350.
Krattinger, S.G. 2009. Molecular characterization of the durable broadspectrum disease resistance gene Lr34 in wheat (Triticum aestivum L.). Universität Zürich, Zürich.
Li, B.; Zhou, Y.; Li, J. & Xie, X. 2010. Diagnosis of diseases by Dr Li Bao Ju: the diagnosis and prevention of tomato Stemphylium leaf spot disease (gray leaf spot). China Veg 24-25.
Lin, T.; Zhu, G.; Zhang, J.; Xu, X.; Yu, Q.; Zheng, Z.; Zhang, Z.; Lun, Y.; Li, S.; Wang, X.; Huang, Z.; Li, J.; Zhang, C.; Wang, T.; Zhang, Y.; Wang, A.; Zhang, Y.; Lin, K.; Li, C.; Xiong, G.; Xue, Y.; Mazzucato, A.; Causse M.; Fei, Z.; Giovannoni, J.J.; Chetelat, R.T.; Zamir, D.; Stadler, T.; Li, J.;Ye, Z.; Du, Y. & Huang, S. 2014. Genomic analyses provide insights into the history of tomato breeding. Nature Genetics 46: 1220-1226
Miranda, B.E.C. & Bolteux, L.S. 2010. Identification REISA. Of Solanum (section lycopersicon) accessions with resistance to Stemphylium solani and S. Lycopersici. Horticultura Brasileira 28: 178-184.
Nasehi, A.; Kadir, J.B.; Esfahani, M.N.; Mahmodi, F.; Ghadirian, H.; Abed Ashtiani, F. & Golkhandan, E. 2013. An outbreak of leaf spot caused by Stemphylium solani on eggplant in Malaysia. Plant Disease 97: 689
Nasehi, A.; Kadir, J.B.; Zainal Abidin, M.A.; Wong, M.Y. & Abed Ashtiani, F. 2012a. First report of gray leaf spot on pepper caused by Stemphylium solani in Malaysia. Plant Disease 96: 1227.
Rani L.; Bai C. & Singh, R.P.2020.Genes, QTLs and linked molecular markers on ferring the resistance for various biotic stresses in tomato cultivar. Journal of Entomology and Zoology Studies 8(1):971-977.
Simmons, E.G. 2001. Perfect states of Stemphylium-IV. Harvard Papers in Botany 199-208.
Su, X.; Zhu, G.; Huang, Z.; Wang, X.; Guo, Y.; Li, B. & Gao, J. 2019. Fine mapping and molecular marker development of the Sm gene conferring resistance to gray leaf spot (Stemphylium spp.) in tomato. Theoretical and Applied Genetics 132(4): 871-882.
Thamer Abdul Hussein Al-Rufaye, Z.; Muhammed Sheriff Al-Sheikh, W. & Kadhum Ghaleb, I .2018. Detection of Lgc1 Gene Low Glutelin Content in Rice CultivarsIraq using Indel Markers. Journal of Global Pharma Technology| 10(08): 193-196.
Yang, H.; Zhao, T.; Jiang, J.; Wang, S.; Wang, A.; Li, J. & Xu, X. 2017. Mapping and screening of the tomato Stemphylium lycopersici resistance gene, Sm, based on bulked segregant analysis in combination with genome resequencing. BMC Plant Biology 17(1): 1-10.
Yang, H.H.; Xu, X.Y.; Zhao, T.T. & Li, J.F. 2017. First Report of Stemphylium lycopersici causing gray Leaf Spot on Eggplant in China. Plant Disease 101(5): 834.
Blancard, D.; Laterrot, H.; Moretti, A.; Pichot, M. 1986. Les Stemphylia rencontrés sur tomate. Phytopathologia Mediterranea 25: 140-144.
Chai, A.L.; Du, G.F.; Shi, Y.X.; Xie, X.W. & Li, B.J. 2015. Leaf spot on sweet potato (Ipomoea batatas) caused by Stemphylium solani, a new disease in China. Journal of Phytopathology 163: 1046-1049.
Cho, H.J.; Kim, B.S. & Hwang, H.S. 2001. Resistance to gray leaf spot in capsicum peppers. Hortscience 36(4): 752-754.
Hendrix, J.W. & Frazier, W.A. 1949. Studies on the inheritance of Stemphylium resistance in tomatoes. Hawaii Agricultural Experiment Station, Technical Bulletin 8.
Kim, B.S.; Cho, H.J.; Hwang, H.S. & Cha, Y.S. 1999. Gray leaf spot of tomato caused by Stephylium solani. The Plant Pathology Journal 15: 348-350.
Krattinger, S.G. 2009. Molecular characterization of the durable broadspectrum disease resistance gene Lr34 in wheat (Triticum aestivum L.). Universität Zürich, Zürich.
Li, B.; Zhou, Y.; Li, J. & Xie, X. 2010. Diagnosis of diseases by Dr Li Bao Ju: the diagnosis and prevention of tomato Stemphylium leaf spot disease (gray leaf spot). China Veg 24-25.
Lin, T.; Zhu, G.; Zhang, J.; Xu, X.; Yu, Q.; Zheng, Z.; Zhang, Z.; Lun, Y.; Li, S.; Wang, X.; Huang, Z.; Li, J.; Zhang, C.; Wang, T.; Zhang, Y.; Wang, A.; Zhang, Y.; Lin, K.; Li, C.; Xiong, G.; Xue, Y.; Mazzucato, A.; Causse M.; Fei, Z.; Giovannoni, J.J.; Chetelat, R.T.; Zamir, D.; Stadler, T.; Li, J.;Ye, Z.; Du, Y. & Huang, S. 2014. Genomic analyses provide insights into the history of tomato breeding. Nature Genetics 46: 1220-1226
Miranda, B.E.C. & Bolteux, L.S. 2010. Identification REISA. Of Solanum (section lycopersicon) accessions with resistance to Stemphylium solani and S. Lycopersici. Horticultura Brasileira 28: 178-184.
Nasehi, A.; Kadir, J.B.; Esfahani, M.N.; Mahmodi, F.; Ghadirian, H.; Abed Ashtiani, F. & Golkhandan, E. 2013. An outbreak of leaf spot caused by Stemphylium solani on eggplant in Malaysia. Plant Disease 97: 689
Nasehi, A.; Kadir, J.B.; Zainal Abidin, M.A.; Wong, M.Y. & Abed Ashtiani, F. 2012a. First report of gray leaf spot on pepper caused by Stemphylium solani in Malaysia. Plant Disease 96: 1227.
Rani L.; Bai C. & Singh, R.P.2020.Genes, QTLs and linked molecular markers on ferring the resistance for various biotic stresses in tomato cultivar. Journal of Entomology and Zoology Studies 8(1):971-977.
Simmons, E.G. 2001. Perfect states of Stemphylium-IV. Harvard Papers in Botany 199-208.
Su, X.; Zhu, G.; Huang, Z.; Wang, X.; Guo, Y.; Li, B. & Gao, J. 2019. Fine mapping and molecular marker development of the Sm gene conferring resistance to gray leaf spot (Stemphylium spp.) in tomato. Theoretical and Applied Genetics 132(4): 871-882.
Thamer Abdul Hussein Al-Rufaye, Z.; Muhammed Sheriff Al-Sheikh, W. & Kadhum Ghaleb, I .2018. Detection of Lgc1 Gene Low Glutelin Content in Rice CultivarsIraq using Indel Markers. Journal of Global Pharma Technology| 10(08): 193-196.
Yang, H.; Zhao, T.; Jiang, J.; Wang, S.; Wang, A.; Li, J. & Xu, X. 2017. Mapping and screening of the tomato Stemphylium lycopersici resistance gene, Sm, based on bulked segregant analysis in combination with genome resequencing. BMC Plant Biology 17(1): 1-10.
Yang, H.H.; Xu, X.Y.; Zhao, T.T. & Li, J.F. 2017. First Report of Stemphylium lycopersici causing gray Leaf Spot on Eggplant in China. Plant Disease 101(5): 834.