Main Article Content
Abstract
The genetic polymorphism of insulin-like growth factor binding protein (IGFBP-3) and its association with the growth traits namely condition factor, body weight, and total length were investigated in common carp Cyprinus carpio using PCR- single strand conformation polymorphism (SSCP) technique. Caudal fin clips from 120 C. carpio, aging 5 months, were used in this study and a 330 bp DNA fragment was amplified from 5'-UTR of carp IGFBP-3. SSCP genotyping yielded seven different banding patterns namely A, B, C, D, E, F, and G with frequencies of 14.16, 12.5, 21.67, 9.17, 11.67, 10.0, and 20.84, respectively. The obtained results showed a relatively high polymorphic behavior of 5'-UTR regulatory region of studied locus; therefore it shows the importance of this region in regulation of transcription of IGFBP-3 gene in carp. Marker-trait association showed significant relationships between the detected genotypes and condition factor (CF). IGFBP-3 gene polymorphism in joint with CF signifies relatively high genetic diversity and significant potential for marker-assisted selection in this species.
Keywords
Common carp
Genetic variations
Growth traits
IGFBP-3.
Article Details
License
The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
How to Cite
MEHRABI, F., KHALESI, M. K., & FARHADI, A. (2015). Polymorphism of insulin-like growth factor binding protein-3 (IGFBP-3) gene associated with growth traits in Cyprinus carpio. Iranian Journal of Ichthyology, 2(3), 172–176. https://doi.org/10.22034/iji.v2i3.43
References
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Almuly, R.; Poleg-Danin, Y.; Gorshkov, S.; Gorshkova, G.; Rapoport B.; Soller, M.; Kashi, Y. & Funkenstein, B. 2005. Characterization of the 5' flanking region of the growth hormone gene of the marine teleost, gilthead sea bream Sparus aurata: Analysis of a polymorphic microsatellite in the proximal promoter. Fisheries Science 71: 479–490.
Baxter, R.C. & Martin, J.L. 1989. Binding proteins for the insulin-like growth factors: structure, regulation and function. Progress in Growth Factors Research 1: 49–68.
Clemmons, D.R.; Dehoff, M.; McCusker, R.; Elgin, R. & Busby, W. 1987. The role of insulin-like growth factor I in the regulation of growth. Journal of Animal Sciences 65: 168-179.
de Bakker, P.I.; Yelensky, R.; Péer, I.; Gabriel, S.B.; Daly, M.J. & Altshuler, D. 1987. Efficiency and power in genetic association studies. Nature Genetics X 37: 1217-1223.
Duan, C.; Ren, H. & Gao, S. 2010. Insulin-like growth factors (IGFs), IGF receptors and IGF-binding proteins: Roles in skeletal muscle growth and differentiation. Genetic and Comparative Physiology 167: 344-351.
Feng, X.; Yu, X. & Tong, J. 2014. Novel single nucleotide polymorphisms of the insulin-like growth factor-I gene and their associations with growth traits in common carp (Cyprinus carpio L.). International Journal of Molecular Sciences 15: 22471–22482.
Hu, G.F. 2010. Ying Lei heterosis and related preliminary study of molecular biology. MSc thesis. Huazhong Agricultural University, Hong Kong.
Jalil Sarqaleh, A.; Moradi Sharbabak, H.; Amini, H.R. & Khalghi, M. 2014. The contribution of major genes to production and economic traits in goats. Genetica in the Third Millennium 2: (In Farsi)
Johnston, I.A.; Bower, N.I. & Macqueen, D.J. 2011. Growth and the regulation of myotomal muscle mass in teleost fish. Journal of Experimental Biology 214: 1617-1628.
Liu, L.S.; Yu, X.M & Tong, J.G. 2012. Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis). Molecular Biology Reports 39: 9211-9221.
Missohou, A.; Talaki, E. & Laminou, I.M. 2006. Diversity and genetic relationships among seven West African goat breeds, Asian Australian Journal of Animal Science 19: 1245.
Nguyen, M.T.; Barnes, A.C.; Mather, P.B.; Li, Y.T. & Lyons, R.E. 2010. Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii. Aquaculture 301: 7–15.
Park, R.; Shepherd, B.S.; Nishioka, R.S.; Grau, E.G. & Bern, H.A. 2000. Effects of homologous pituitary hormone treatment on serum insulin-like growth-factor-binding proteins (IGFBPs) in hypophysectomized tilapia, Oreochromis mossambicus, with special reference to a novel 20-kDa IGFBP. Genetic and Comparative Endocrinology 117: 404–412.
Ren, H.; Accili, D. & Duan, C. 2010. Hypoxia converts the myogenic action of insulin-like growth factors into mitogenic action by differentially regulating multiple signaling pathways, Proceedings of the National Academy of Science USA 107: 5857-5862.
Sun, Y.; Yu, X. & Tong, J. 2012. Polymorphisms in myostatin gene and associations with growth traits in the common carp (Cyprinus carpio L.). International Journal of Molecular Sciences 13: 14956-14961.
Tabor, H.K.; Risch, N.J. & Myers, R.M. 2002. Candidate-gene approaches for studying complex genetic traits: practical considerations. Nature Reviews in Genetics 3: 391-397.
Tambasco, D.D.; Paz, C.C. & Tambasco‐Studart, M. 2003. Candidate genes for growth traits in beef cattle crosses Bos taurus × Bos indicus. Animal Breeding and Genetics 120: 51-56.
Tian, C.; Yang, M.; Lv, L.; Yuan, Y.; Liang, X.; Guo, W.; Song, Y. & Zhao, C. 2014. Single nucleotide polymorphisms in growth hormone gene and their association with growth traits in Siniperca chuatsi (Basilewsky). International Journal of Molecular Sciences 15: 7029–7036.
Van-Arendonk, J.A.M.; Tier, B. & Kinghorn, B.P. 1994. Use of multiple genetic markers in prediction of breeding values. Genetics 137: 319-329.
References
Almuly, R.; Poleg-Danin, Y.; Gorshkov, S.; Gorshkova, G.; Rapoport B.; Soller, M.; Kashi, Y. & Funkenstein, B. 2005. Characterization of the 5' flanking region of the growth hormone gene of the marine teleost, gilthead sea bream Sparus aurata: Analysis of a polymorphic microsatellite in the proximal promoter. Fisheries Science 71: 479–490.
Baxter, R.C. & Martin, J.L. 1989. Binding proteins for the insulin-like growth factors: structure, regulation and function. Progress in Growth Factors Research 1: 49–68.
Clemmons, D.R.; Dehoff, M.; McCusker, R.; Elgin, R. & Busby, W. 1987. The role of insulin-like growth factor I in the regulation of growth. Journal of Animal Sciences 65: 168-179.
de Bakker, P.I.; Yelensky, R.; Péer, I.; Gabriel, S.B.; Daly, M.J. & Altshuler, D. 1987. Efficiency and power in genetic association studies. Nature Genetics X 37: 1217-1223.
Duan, C.; Ren, H. & Gao, S. 2010. Insulin-like growth factors (IGFs), IGF receptors and IGF-binding proteins: Roles in skeletal muscle growth and differentiation. Genetic and Comparative Physiology 167: 344-351.
Feng, X.; Yu, X. & Tong, J. 2014. Novel single nucleotide polymorphisms of the insulin-like growth factor-I gene and their associations with growth traits in common carp (Cyprinus carpio L.). International Journal of Molecular Sciences 15: 22471–22482.
Hu, G.F. 2010. Ying Lei heterosis and related preliminary study of molecular biology. MSc thesis. Huazhong Agricultural University, Hong Kong.
Jalil Sarqaleh, A.; Moradi Sharbabak, H.; Amini, H.R. & Khalghi, M. 2014. The contribution of major genes to production and economic traits in goats. Genetica in the Third Millennium 2: (In Farsi)
Johnston, I.A.; Bower, N.I. & Macqueen, D.J. 2011. Growth and the regulation of myotomal muscle mass in teleost fish. Journal of Experimental Biology 214: 1617-1628.
Liu, L.S.; Yu, X.M & Tong, J.G. 2012. Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis). Molecular Biology Reports 39: 9211-9221.
Missohou, A.; Talaki, E. & Laminou, I.M. 2006. Diversity and genetic relationships among seven West African goat breeds, Asian Australian Journal of Animal Science 19: 1245.
Nguyen, M.T.; Barnes, A.C.; Mather, P.B.; Li, Y.T. & Lyons, R.E. 2010. Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii. Aquaculture 301: 7–15.
Park, R.; Shepherd, B.S.; Nishioka, R.S.; Grau, E.G. & Bern, H.A. 2000. Effects of homologous pituitary hormone treatment on serum insulin-like growth-factor-binding proteins (IGFBPs) in hypophysectomized tilapia, Oreochromis mossambicus, with special reference to a novel 20-kDa IGFBP. Genetic and Comparative Endocrinology 117: 404–412.
Ren, H.; Accili, D. & Duan, C. 2010. Hypoxia converts the myogenic action of insulin-like growth factors into mitogenic action by differentially regulating multiple signaling pathways, Proceedings of the National Academy of Science USA 107: 5857-5862.
Sun, Y.; Yu, X. & Tong, J. 2012. Polymorphisms in myostatin gene and associations with growth traits in the common carp (Cyprinus carpio L.). International Journal of Molecular Sciences 13: 14956-14961.
Tabor, H.K.; Risch, N.J. & Myers, R.M. 2002. Candidate-gene approaches for studying complex genetic traits: practical considerations. Nature Reviews in Genetics 3: 391-397.
Tambasco, D.D.; Paz, C.C. & Tambasco‐Studart, M. 2003. Candidate genes for growth traits in beef cattle crosses Bos taurus × Bos indicus. Animal Breeding and Genetics 120: 51-56.
Tian, C.; Yang, M.; Lv, L.; Yuan, Y.; Liang, X.; Guo, W.; Song, Y. & Zhao, C. 2014. Single nucleotide polymorphisms in growth hormone gene and their association with growth traits in Siniperca chuatsi (Basilewsky). International Journal of Molecular Sciences 15: 7029–7036.
Van-Arendonk, J.A.M.; Tier, B. & Kinghorn, B.P. 1994. Use of multiple genetic markers in prediction of breeding values. Genetics 137: 319-329.
Baxter, R.C. & Martin, J.L. 1989. Binding proteins for the insulin-like growth factors: structure, regulation and function. Progress in Growth Factors Research 1: 49–68.
Clemmons, D.R.; Dehoff, M.; McCusker, R.; Elgin, R. & Busby, W. 1987. The role of insulin-like growth factor I in the regulation of growth. Journal of Animal Sciences 65: 168-179.
de Bakker, P.I.; Yelensky, R.; Péer, I.; Gabriel, S.B.; Daly, M.J. & Altshuler, D. 1987. Efficiency and power in genetic association studies. Nature Genetics X 37: 1217-1223.
Duan, C.; Ren, H. & Gao, S. 2010. Insulin-like growth factors (IGFs), IGF receptors and IGF-binding proteins: Roles in skeletal muscle growth and differentiation. Genetic and Comparative Physiology 167: 344-351.
Feng, X.; Yu, X. & Tong, J. 2014. Novel single nucleotide polymorphisms of the insulin-like growth factor-I gene and their associations with growth traits in common carp (Cyprinus carpio L.). International Journal of Molecular Sciences 15: 22471–22482.
Hu, G.F. 2010. Ying Lei heterosis and related preliminary study of molecular biology. MSc thesis. Huazhong Agricultural University, Hong Kong.
Jalil Sarqaleh, A.; Moradi Sharbabak, H.; Amini, H.R. & Khalghi, M. 2014. The contribution of major genes to production and economic traits in goats. Genetica in the Third Millennium 2: (In Farsi)
Johnston, I.A.; Bower, N.I. & Macqueen, D.J. 2011. Growth and the regulation of myotomal muscle mass in teleost fish. Journal of Experimental Biology 214: 1617-1628.
Liu, L.S.; Yu, X.M & Tong, J.G. 2012. Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis). Molecular Biology Reports 39: 9211-9221.
Missohou, A.; Talaki, E. & Laminou, I.M. 2006. Diversity and genetic relationships among seven West African goat breeds, Asian Australian Journal of Animal Science 19: 1245.
Nguyen, M.T.; Barnes, A.C.; Mather, P.B.; Li, Y.T. & Lyons, R.E. 2010. Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii. Aquaculture 301: 7–15.
Park, R.; Shepherd, B.S.; Nishioka, R.S.; Grau, E.G. & Bern, H.A. 2000. Effects of homologous pituitary hormone treatment on serum insulin-like growth-factor-binding proteins (IGFBPs) in hypophysectomized tilapia, Oreochromis mossambicus, with special reference to a novel 20-kDa IGFBP. Genetic and Comparative Endocrinology 117: 404–412.
Ren, H.; Accili, D. & Duan, C. 2010. Hypoxia converts the myogenic action of insulin-like growth factors into mitogenic action by differentially regulating multiple signaling pathways, Proceedings of the National Academy of Science USA 107: 5857-5862.
Sun, Y.; Yu, X. & Tong, J. 2012. Polymorphisms in myostatin gene and associations with growth traits in the common carp (Cyprinus carpio L.). International Journal of Molecular Sciences 13: 14956-14961.
Tabor, H.K.; Risch, N.J. & Myers, R.M. 2002. Candidate-gene approaches for studying complex genetic traits: practical considerations. Nature Reviews in Genetics 3: 391-397.
Tambasco, D.D.; Paz, C.C. & Tambasco‐Studart, M. 2003. Candidate genes for growth traits in beef cattle crosses Bos taurus × Bos indicus. Animal Breeding and Genetics 120: 51-56.
Tian, C.; Yang, M.; Lv, L.; Yuan, Y.; Liang, X.; Guo, W.; Song, Y. & Zhao, C. 2014. Single nucleotide polymorphisms in growth hormone gene and their association with growth traits in Siniperca chuatsi (Basilewsky). International Journal of Molecular Sciences 15: 7029–7036.
Van-Arendonk, J.A.M.; Tier, B. & Kinghorn, B.P. 1994. Use of multiple genetic markers in prediction of breeding values. Genetics 137: 319-329.