Main Article Content


The genetic polymorphism of insulin-like growth factor binding protein (IGFBP-3) and its association with the growth traits namely condition factor, body weight, and total length were investigated in common carp Cyprinus carpio using PCR- single strand conformation polymorphism (SSCP) technique. Caudal fin clips from 120 C. carpio, aging 5 months, were used in this study and a 330 bp DNA fragment was amplified from 5'-UTR of carp IGFBP-3. SSCP genotyping yielded seven different banding patterns namely A, B, C, D, E, F, and G with frequencies of 14.16, 12.5, 21.67, 9.17, 11.67, 10.0, and 20.84, respectively. The obtained results showed a relatively high polymorphic behavior of 5'-UTR regulatory region of studied locus; therefore it shows the importance of this region in regulation of transcription of IGFBP-3 gene in carp.  Marker-trait association showed significant relationships between the detected genotypes and condition factor (CF). IGFBP-3 gene polymorphism in joint with CF signifies relatively high genetic diversity and significant potential for marker-assisted selection in this species.


Common carp Genetic variations Growth traits IGFBP-3.

Article Details

How to Cite
MEHRABI, F., KHALESI, M. K., & FARHADI, A. (2015). Polymorphism of insulin-like growth factor binding protein-3 (IGFBP-3) gene associated with growth traits in Cyprinus carpio. Iranian Journal of Ichthyology, 2(3), 172–176.


    Almuly, R.; Poleg-Danin, Y.; Gorshkov, S.; Gorshkova, G.; Rapoport B.; Soller, M.; Kashi, Y. & Funkenstein, B. 2005. Characterization of the 5' flanking region of the growth hormone gene of the marine teleost, gilthead sea bream Sparus aurata: Analysis of a polymorphic microsatellite in the proximal promoter. Fisheries Science 71: 479–490.
    Baxter, R.C. & Martin, J.L. 1989. Binding proteins for the insulin-like growth factors: structure, regulation and function. Progress in Growth Factors Research 1: 49–68.
    Clemmons, D.R.; Dehoff, M.; McCusker, R.; Elgin, R. & Busby, W. 1987. The role of insulin-like growth factor I in the regulation of growth. Journal of Animal Sciences 65: 168-179.
    de Bakker, P.I.; Yelensky, R.; Péer, I.; Gabriel, S.B.; Daly, M.J. & Altshuler, D. 1987. Efficiency and power in genetic association studies. Nature Genetics X 37: 1217-1223.
    Duan, C.; Ren, H. & Gao, S. 2010. Insulin-like growth factors (IGFs), IGF receptors and IGF-binding proteins: Roles in skeletal muscle growth and differentiation. Genetic and Comparative Physiology 167: 344-351.
    Feng, X.; Yu, X. & Tong, J. 2014. Novel single nucleotide polymorphisms of the insulin-like growth factor-I gene and their associations with growth traits in common carp (Cyprinus carpio L.). International Journal of Molecular Sciences 15: 22471–22482.
    Hu, G.F. 2010. Ying Lei heterosis and related preliminary study of molecular biology. MSc thesis. Huazhong Agricultural University, Hong Kong.
    Jalil Sarqaleh, A.; Moradi Sharbabak, H.; Amini, H.R. & Khalghi, M. 2014. The contribution of major genes to production and economic traits in goats. Genetica in the Third Millennium 2: (In Farsi)
    Johnston, I.A.; Bower, N.I. & Macqueen, D.J. 2011. Growth and the regulation of myotomal muscle mass in teleost fish. Journal of Experimental Biology 214: 1617-1628.
    Liu, L.S.; Yu, X.M & Tong, J.G. 2012. Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis). Molecular Biology Reports 39: 9211-9221.
    Missohou, A.; Talaki, E. & Laminou, I.M. 2006. Diversity and genetic relationships among seven West African goat breeds, Asian Australian Journal of Animal Science 19: 1245.
    Nguyen, M.T.; Barnes, A.C.; Mather, P.B.; Li, Y.T. & Lyons, R.E. 2010. Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii. Aquaculture 301: 7–15.
    Park, R.; Shepherd, B.S.; Nishioka, R.S.; Grau, E.G. & Bern, H.A. 2000. Effects of homologous pituitary hormone treatment on serum insulin-like growth-factor-binding proteins (IGFBPs) in hypophysectomized tilapia, Oreochromis mossambicus, with special reference to a novel 20-kDa IGFBP. Genetic and Comparative Endocrinology 117: 404–412.
    Ren, H.; Accili, D. & Duan, C. 2010. Hypoxia converts the myogenic action of insulin-like growth factors into mitogenic action by differentially regulating multiple signaling pathways, Proceedings of the National Academy of Science USA 107: 5857-5862.
    Sun, Y.; Yu, X. & Tong, J. 2012. Polymorphisms in myostatin gene and associations with growth traits in the common carp (Cyprinus carpio L.). International Journal of Molecular Sciences 13: 14956-14961.
    Tabor, H.K.; Risch, N.J. & Myers, R.M. 2002. Candidate-gene approaches for studying complex genetic traits: practical considerations. Nature Reviews in Genetics 3: 391-397.
    Tambasco, D.D.; Paz, C.C. & Tambasco‐Studart, M. 2003. Candidate genes for growth traits in beef cattle crosses Bos taurus × Bos indicus. Animal Breeding and Genetics 120: 51-56.
    Tian, C.; Yang, M.; Lv, L.; Yuan, Y.; Liang, X.; Guo, W.; Song, Y. & Zhao, C. 2014. Single nucleotide polymorphisms in growth hormone gene and their association with growth traits in Siniperca chuatsi (Basilewsky). International Journal of Molecular Sciences 15: 7029–7036.
    Van-Arendonk, J.A.M.; Tier, B. & Kinghorn, B.P. 1994. Use of multiple genetic markers in prediction of breeding values. Genetics 137: 319-329.