Virulence gene expression of Pseudomonas aeruginosa isolated from children with nephrotic syndrome

Zainab Amer HATEM, Adnan Ali HAMMAD, Saade Abdalkareem JASIM

Abstract

Pseudomonas aeruginosa is Gram-negative, considered as the most common opportunistic pathogens which lead to high rate of mortality and morbidity when subject suffered from compromised immune systems. This study was aimed to estimate the lasI and lasR genes expression of P. aeruginosa isolated from children with nephrotic syndrome. Nineteen isolates from urine samples of children with nephropathy syndrome are included in this study and confirmed by VITEK test as a P. aeruginosa strains. The result showed that the LasI and LasR genes showed a high level of gene expression in strains that grown with ceftriaxone than the strains that grown alone, but the gene LasI had a higher level of expression than the LasR gene, also a regression analysis were done to reveal the effect of LasI on the LasR gene which showed a positive effect which mean any increment in the gene expression level of LasI gene will combined with an increment in the LasR gene, (R= 0.899 and P= 0.001).

Keywords

Nephrotic syndrome, LasI and LasR genes, Gene expression.

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References

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