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Abstract

The success of spawning in striped catfish is largely determined by sperm quality, which can be influenced by several factors, including the age of the male broodstock. This study evaluates the influence of broodstock age on critical sperm quality metrics (motility, viability, and abnormalities) and reproductive success (fertilization and hatching rates) in striped catfish. For the treatments, male striped catfish were grouped into four age categories: Group A (1 year), Group B (2-4 years), Group C (5-7 years), and Group D (>8 years).  Sperm volume was measured using a 10mL graduated tube, while sperm concentration, motility, viability, and abnormality were assessed microscopically using the Sperm Class Analyzer SCA® CASA system automatic sperm analyzer. To evaluate sperm performance during fertilization, eggs from the same female were used to assess fertilization rate, hatching rate, and larval abnormalities. The findings showed that male striped catfish aged 2-4 and 5-7 years demonstrated the most favorable sperm quality, with an average sperm volume of 51.0mL, concentration of 29.9million/mL, motility of 96.4%, viability of 97.4%, and abnormality rate of 3.6%. In contrast, the 1-year-old group showed the lowest performance, with an average sperm volume of 11.5mL, concentration of 27.7million/mL, motility of 66.5%, viability of 67.4%, and abnormality rate of 1.4%. Based on these results, it is recommended that broodstock aged 2-4 years be prioritized for spawning activities due to their higher sperm production, better sperm quality and they produce offspring that have good survival.

Keywords

Pangasius catfish Sperm Class Analyzer SCA motility Reproduction

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

How to Cite
DARMAWAN, J., TAHAPARI, E., PAMUNGKAS, W., SUHARYANTO, SUBANGKIT, A. T., ASTUTI, D. N., BARADES, E., & ABDURACHMAN, M. L. (2025). Sperm quality and quantity in striped catfish, <i>Pangasianodon hypophthalmus</i> (Sauvage 1878) at various broodstock age stages. Iranian Journal of Ichthyology, 12(2), 113–122. https://doi.org/10.22034/iji.v12i2.1051
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References

  1. De Almeida-Monteiro, P.S.; Pinheiro, R.R.R.; Oliveira-Araújo, M.S.; Sales, Y.S.; do Nascimento, R.V.; Nunes, L.T.; Pereira, V.A.; Montenegro, A.R.; Melo-Maciel, M.A.P. & Salmito-Vanderley, C.S.B. 2020. Sperm vitrification of Prochilodus brevis using Powder Coconut Water (ACP-104) in association with different cryoprotectant concentrations. Aquaculture Research 51(11): 4565-4574.
  2. Betsy, C.J.; Sampath Kumar, J.S.; Pon Jawahar, K.T.; Athithan, S.; Ahilan, B. & Karal Marx, K. 2016. Captive broodstock development of common carp Cyprinus carpio Linnaeus, 1758: influence of age on spermatogenesis and fertilisation parameters. Indian Journal of Fisheries 63(4): 75-81.63.
  3. Boryshpolets, S.; Kowalski, R.K.; Dietrich, G.J.; Dzyuba, B. & Ciereszko, A. 2013. Different computer-assisted sperm analysis (CASA) systems highly influence sperm motility parameters. Theriogenology 80(7): 758-765.
  4. Casselman, S.J.; Schulte-Hostedde, A. & Montgomerie, R. 2006. Sperm quality influences fertilization success in walleye (Sander vitreus). Canadian Journal of Fisheries and Aquatic Sciences 63(9): 2119-2125.
  5. Chalde, T.; Elisio, M. & Miranda, L.A. 2014. Quality of pejerrey (Odontesthes bonariensis) eggs and larvae in captivity throughout spawning season. Neotropical Ichthyology 12(3): 629-634.
  6. Erraud, A.; Bonnard, M.; Cornet, V.; Ammar, I.B.; Antipine, S.; Peignot, Q.; Lambert, J.; Mandiki, S.N.M. & Kestemont, P. 2021. How age, captivity and cryopreservation affect sperm quality and reproductive efficiency in precocious Atlantic salmon (Salmo salar L. 1758). Aquaculture 544: 737047.
  7. Evans, J.P.; Pelempar, T.E. & Magurran, A.E. 2002. The ontogeny of courtship, colour and sperm production in male guppies. Journal of Fish Biology 60(2): 495-498.
  8. FAO (Food and Agriculture Organization of the United Nations). 2022. The State of World Fisheries and Aquaculture 2022. Towards Blue Transformation. Rome, FAO. 266 p.
  9. Gasparini, C.; Kelley, J.L. & Evans, J.P. 2014. Male sperm storage compromises sperm motility in guppies. Biology Letters 10: 0681.
  10. Halvaei, I.; Litzky, J. & Esfandiari, N. 2020. Advanced paternal age: effects on sperm parameters, assisted reproduction outcomes and offspring health. Reproductive Biology and Endocrinology 18(1): 110.
  11. Hanjavanit, C.; Kitancharoen, N. & Rakmanee, C. 2008. Experimental infection of aquatic fungi on eggs of African catfish (Clarias gariepinus Burch). Khon Kaen University Science 36: 36-43.
  12. Johnson S.L.; Zellhuber-McMillan S.; Gillum J.; Dunleavy J.; Evans J.P.; Nakagawa S. & Gemmell N.J. 2018. Evidence that fertility trades off with early offspring fitness as males age. Proceedings of the Royal Society B: Biological Science 285(1871): 20172174.
  13. Jory, D. 2023. Global finfish production forecast covers species that will contribute at least 41 percent of the total aquaculture production (live weight, excluding algae) projected for 2023 and 2024. Global Seafood Alliance’s (GSA) 2023. https://www.globalseafood.org/advocate/annual-farmed-finfish-production-survey-a-modest-supply-decline-for-2023-and-a-predicted-return-to-growth-in-2024/
  14. Kanuga, M.K.; Benner, M.J.; Doble, J.A.; Wilson-Leedy, J.G.; Robison, B.D. & Ingermann, R.L. 2011. Effect of aging on male reproduction in zebrafish (Danio rerio). Journal of Experimental Zoology Part A: Ecological Genetics and Physiology 315(3): 156-161.
  15. Kim, S.W.; Hong, W.H.; Han, S.J.; Kwon, J.; Ko, H.; Lee, S.B.; Giri, S.S.; Kim, S.G.; Kim, B.Y.; Jang, G.; Lee, B.C.; Kim, D.W. & Park, S.C. 2020. Use of Synthetic Salmon GnRH and Domperidone (Ovaprim®) in Sharks: Preparation for ex situ Conservation. Frontiers in Marine Science 7: 571741.
  16. KKP (Ministry of Marine Affairs and Fisheries of the Republic of Indonesia). 2024. Static Table: Production Volume of Aquaculture for Rearing per Main Commodity (Tons). https://portaldata.kkp.go.id/portals/data-statistik/prod-ikan/tbl-statis/d/53
  17. Kholodnyy, V.; Gadêlha, H.; Cosson, J. & Boryshpolets, S. 2020. How do freshwater fish sperm find the egg? The physicochemical factors guiding the gamete encounters of externally fertilizing freshwater fish. Reviews in Aquaculture 12(2): 1165-1192.
  18. Kowalski, R. & Cejko, B. 2019. Sperm quality in fish: Determinants and affecting factors. Theriogenology 135: 94-108.
  19. Kurta, K.; Jeuthe, H.; Naboulsi, R.; de Koning, D.J. & Palaiokostas, C. 2023. Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus). BMC Genomics 24: 519.
  20. Mylonas, C.C.; Fostier, A. & Zanuy, S. 2010. Broodstock management and hormonal manipulations of fish reproduction. General and comparative endocrinology 165(3): 516-534.
  21. Otoh, A. & Udoh, J. 2018. Age-related sperm quality of male Heterobranchus longifilis broodstock fed two different isonitrogenous feeds. Tropical Freshwater Biology 27: 31.
  22. Pamungkas, W.; Darmawan, J. & Khasani I. 2023. The effect of different diets on the quality of sperm in striped catfish (Pangasianodon hypophthalmus). AACL Bioflux 16(4): 2166-2173
  23. Riesco, M.F.; Valcarce, D.G.; Martínez-Vázquez, J.M. & Robles V. 2019. Effect of low sperm quality on progeny: a study on zebrafish as model species. Scientific Reports 9(1): 11192.
  24. Risopatrón, J.; Merino, O.; Cheuquemán, C.; Figueroa, E.; Sánchez, R.; Farías J.G. & Valdebenito, I. 2018. Effect of the age of broodstock males on sperm function during cold storage in the trout (Oncorhynchus mykiss). Andrologia 50(2): e12857.
  25. Schmidt H. & Kamp G. 2004. Induced hyperactivity in boar spermatozoa and its evaluation by computer-assisted sperm analysis. Reproduction 128(2): 171-179.
  26. Seyedi, J.; Kalbassi, M.R.; Esmaeilbeigi, M.; Tayemeh, M.B. & Moghadam, J.A. 2021. Toxicity and deleterious impacts of selenium nanoparticles at supranutritional and imbalance levels on male goldfish (Carassius auratus) sperm. Journal of Trace Elements in Medicine and Biology 66: 126758.
  27. Shamspour, S. & Khara, H. 2016. Effect of age on reproductive efficiency of adult rainbow trout, Oncorhynchus mykiss Walbaum, 1972. Iranian Journal of Fisheries Sciences 15(3): 945-956.
  28. Shazada, N.E.; Alavi, S.M.H.; Siddique, M.A.M.; Cheng, Y.; Zhang, S.; Rodina, M.; Kocour M. & Linhart O. 2024. Short-term storage of sperm in common carp from laboratory research to commercial production - A review. Reviews in Aquaculture 16(1): 174-189. 12827.
  29. Tilahun, G.; Dube, K.; Chtruvedi, C.S. & Kumar B. 2016. Assessment of reproductive performance, growth and survival of hybrids of African catfish (Clarias gariepinus) and Indian catfish (Clarias batrachus) compared to their parental lines crosses. Turkish Journal Fisheries and Aquatic Science 16(1): 123-133.
  30. Woo, S.M.; Lee, H.B.; Seo, Y.S. & Lim, H.K. 2021. Effects of exogenous hormones treatment on spermiation and plasma levels of gonadal steroids in Roughscale sole, Clidoderma asperrimum Fisheries and Aquatic Sciences 24(12): 437-445.
  31. Vuthiphandchai V. & Zohar, Y. 1999. Age-related sperm quality of captive striped bass Morone saxatilis. Journal of the World Aquaculture Society 30(1): 65-72.
  32. Yanong R.P.E.; Martinez, C. & Watson, C.A. 2010. Use of Ovaprim in Ornamental Fish Aquaculture: FA161/FA161, 12/2009. EDIS 2010 (2).