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Abstract

The Acipenseridae is a crucial inhabitant of aquatic ecosystems, and environmental adversity can have harmful consequences on their health. Stress-relieving compounds and immune system stimulants are currently used to support fish resistance. This study aimed to investigate the metabolic and immune responses to thermal stress in starry sturgeon juvenile previously exposed to amygdalin as a stress-reducing compound. The juveniles were treated at different temperatures (18°C, 22°C, and 26°C) with or without amygdalin, with three replicates. Blood and liver tissue samples were collected, and various parameters were measured. The results revealed significant differences in serum enzymes, cortisol levels, antioxidant capacity, and immune system components among the different groups. It was evident that fish pre-treated with amygdalin and kept at 22 °C (A+T22) and 26 °C (A+T26) experienced a significant decline in ALT (alanine aminotransferase, AST (aspartate aminotransferase) and T-AOC (Total Antioxidant Capacity) levels compared to those who did not undergo such treatment (fish kept at 22 and 26°C). Moreover, when comparing amygdalin-exposed fish at 26°C with their counterparts who were not exposed to amygdalin, there was a noticeable decrease in ALP and cortisol levels. Thermal stress has an adverse effect on the immune parameters of starry sturgeon. However, when amygdalin is administered, it has the potential to enhance fish resilience against thermal stressors by increasing immunity, particularly in C3 and IgM. In conclusion, amygdalin could serve as a beneficial agent for fish by mitigating stress levels and enhancing their resilience against thermal stressors.

Keywords

Metabolic response Sturgeon Immunity Stress

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The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

How to Cite
KOUCHAKI, H., HEIDARI, B., HADAVI, M., & SERVILI, A. (2025). Physiological responses of Starry Sturgeon (<i>Acipenser stellatus</i>) juvenile to thermal stress: exploring the effects of Amygdalin in enhancing Sturgeon resilience. Iranian Journal of Ichthyology, 12(1), 22–38. https://doi.org/10.22034/iji.v12i1.1039
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References

  1. Abdelhadya, D.H.; El-Magd, M.A.; Elbialy, Z.I. & Saleh, A.A. 2017. Bromuconazole-induced hepatotoxicity is accompanied by upregulation of PXR/CYP3A1 and downregulation of CAR/CYP2B1 gene expression. Toxicology Mechanisms and Methods 27(7):544-550.
  2. Achikanu, C.E. & Ani, O.N. 2021. Effect of sublethal dose of dichlorvos on the biochemical parameters of African catfish. Journal of Biochemistry and Analytical Studies 5(1).
  3. Alexander, C.; Sahu, N.P.; Pal, A.K. & Akhtar, M.S. 2011. Haemato‐immunological and stress responses of Labeo rohita (Hamilton) fingerlings: effect of rearing temperature and dietary gelatinized carbohydrate. Journal of Animal Physiology and Animal Nutrition 95(5): pp.653-663.
  4. Al-Khshali, M.S. & Al Hilali, H.A. 2019. Some physiological changes (ALP, AST and ALT) of common carp (Cyprinus carpio) caused by high salinity. Biochemical & Cellular Archives 19(2).
  5. Alwan, A.M.; Rokaya, D.; Kathayat, G. & Afshari, J.T. 2023. Onco-immunity and therapeutic application of amygdalin: A review. Journal of Oral Biology and Craniofacial Research 13(2): 155-163.
  6. Amjad, S.; Sharma, A.K. & Serajuddin, M. 2018. Toxicity assessment of cypermethrin nanoparticles in Channa punctatus: Behavioural response, micronuclei induction and enzyme alteration. Regulatory Toxicology and Pharmacology 100: 127-133.
  7. Bacalbasa-Dobrovici, N. 1997. Endangered migratory sturgeons of the lower Danube River and its delta. Sturgeon Biodiversity and Conservation, pp. 201-207.
  8. Barakat, H., Aljutaily, T., Almujaydil, M.S., Algheshairy, R.M., Alhomaid, R.M., Almutairi, A.S.; Alshimali, S.I. & Abdellatif, A.A. 2022. Amygdalin: a review on its characteristics, antioxidant potential, gastrointestinal microbiota intervention, anticancer therapeutic and mechanisms, toxicity, and encapsulation. Biomolecules 12(10): 1514.
  9. Barbosa, L.M.G.; Moraes, G.; de Freitas Anibal, F. & Marzocchi-Machado, C.M. 2020. Effect of environmental thermal fluctuations on innate immune responses in pacu Piaractus mesopotamicus juveniles. Aquaculture Reports 17: 100303.
  10. Barton, B.A. 2000. Salmonid fishes differ in their cortisol and glucose responses to handling and transport stress. North American Journal of Aquaculture 62(1): 12-18.
  11. Barton, B.A.; Ribas, L.; Acerete, L. & Tort, L. 2005. Effects of chronic confinement on physiological responses of juvenile gilthead sea bream, Sparus aurata L., to acute handling. Aquaculture Research, 36(2): 72-179.
  12. Berger, R. 1984. Methods of enzymatic analysis . Volume II “Samples, Reagents, Assessment of Results”. Weinheim/Deerfield Beach, Florida/Basel: Verlag Chemie 1983. 539 S., 37 Abb., 21 Tab., 215 DM (wenn alle Bände), 245 DM (Einzelband).
  13. Bowden, T.J. 2008. Modulation of the immune system of fish by their environment. Fish & Shellfish Immunology 25(4): 373-383.
  14. Boyer, S.E.; White, J.S.,; Stier, A.C. & Osenberg, C.W. 2009. Effects of the fish anesthetic, clove oil (eugenol), on coral health and growth. Journal of Experimental Marine Biology and Ecology 369(1): 53-57.
  15. Cascarano, M.C.; Stavrakidis-Zachou, O.; Mladineo, I.; Thompson, K.D.; Papandroulakis, N. & Katharios, P. 2021. Mediterranean aquaculture in a changing climate: Temperature effects on pathogens and diseases of three farmed fish species. Pathogens 10(9): 1205.
  16. Chen, Y.U.; Ma, J.; Wang, F.; Hu, J.; Cui, A.; Wei, C.; Yang, Q. & Li, F. 2013. Amygdalin induces apoptosis in human cervical cancer cell line HeLa cells. Immunopharmacology and Immunotoxicology 35(1): 43-51.
  17. Cheng, C.H.; Ye, C.X.; Guo, Z.X. & Wang, A.L. 2017. Immune and physiological responses of pufferfish (Takifugu obscurus) under cold stress. Fish & Shellfish Immunology 64: 137-145.
  18. Choresh, O.; Loya, Y.; Müller, W.E.; Wiedenmann, J. & Azem, A. 2004. The mitochondrial 60-kDa heat shock protein in marine invertebrates: biochemical purification and molecular characterization. Cell Stress & Chaperones 9(1): 38.
  19. Christybapita, D.; Divyagnaneswari, M. & Michael, R.D. 2007. Oral administration of Eclipta alba leaf aqueous extract enhances the non-specific immune responses and disease resistance of Oreochromis mossambicus. Fish & Shellfish Immunology 23(4): 840-852.
  20. Cipollone, R.; Ascenzi, P.; Tomao, P.; Imperi, F. & Visca, P. 2008. Enzymatic detoxification of cyanide: clues from Pseudomonas aeruginosa Rhodanese. Journal of Molecular Microbiology and Biotechnology 15(2-3): 199-211.
  21. Corson, T.W. & Crews, C.M. 2007. Molecular understanding and modern application of traditional medicines: triumphs and trials. Cell 130(5): 769-774.
  22. Danijela, K.I.R.O.V.S.K.I. 2015. Endocrine and metabolic adaptations of calves to extra-uterine life. Acta Veterinaria 65(3): 297-318.
  23. Das, D.N. & Panda, P.K. 2020. Role of Xenobiotic in Autophagy Inflection in Cell Death and Carcinogenesis. Autophagy in Tumor and Tumor Microenvironment 1-34.
  24. Dawood, M.A.; Metwally, A.E.S.; El-Sharawy, M.E.; Ghozlan, A.M.; Abdel-Latif, H.M.; Van Doan, H. & Ali, M.A. 2020. The influences of ferulic acid on the growth performance, haemato-immunological responses, and immune-related genes of Nile tilapia (Oreochromis niloticus) exposed to heat stress. Aquaculture 525: 735320.
  25. Dettleff, P.; Zuloaga, R.; Fuentes, M.; Gonzalez, P.; Aedo, J.; Estrada, J.M.; Molina, A. & Valdés, J.A. 2022. High-temperature stress effect on the red cusk-eel (Geypterus chilensis) liver: transcriptional modulation and oxidative stress damage. Biology 11(7): 990.
  26. Dominguez, M.; Takemura, A.; Tsuchiya, M. & Nakamura, S. 2004. Impact of different environmental factors on the circulating immunoglobulin levels in the Nile tilapia, Oreochromis niloticus. Aquaculture 241(1-4): 491-500.
  27. Dudu, A.; Georgescu, S.E.; LUCA, C.; Suciu, R. & Costache, M. 2008. Microsatelitte DNA variation in the black sea stellate sturgeon, Acipenser stellatus. Scientific Papers Animal Science and Biotechnologies 41(1): 78-78.
  28. Elsayed, Y.Y.; Kuhl, T.; Dauer, K.; Sayin, A.; Wagner, K.G. & Imhof, D. 2022. Bioanalytical workflow for qualitative and quantitative assessment of hot-melt extruded lysozyme formulations. ACS Omega 7(45): 40836-40843.
  29. Garcia, A.L.; Rühl, R.; Herz, U.; Koebnick, C.; Schweigert, F.J. & Worm, M. 2003. Retinoid‐and carotenoid‐enriched diets influence the ontogenesis of the immune system in mice. Immunology 110(2): 180-187.
  30. Goikoetxea, A.; Sadoul, B.; Blondeau-Bidet, E.; Aerts, J.; Blanc, M.O.; Parrinello, H.; Barrachina, C.; Pratlong, M. & Geffroy, B. 2021. Genetic pathways underpinning hormonal stress responses in fish exposed to short-and long-term warm ocean temperatures. Ecological Indicators 120: 106937.
  31. Goos, H.T. & Consten, D. 2002. Stress adaptation, cortisol and pubertal development in the male common carp, Cyprinus carpio. Molecular and Cellular Endocrinology 197(1-2): 105-116.
  32. Herrera, M.; Mancera, J.M. & Costas, B. 2019. The use of dietary additives in fish stress mitigation: comparative endocrine and physiological responses. Frontiers in Endocrinology 10: 447.
  33. Honda, M. & Suzuki, N. 2020. Toxicities of polycyclic aromatic hydrocarbons for aquatic animals. International Journal of Environmental Research and Public Health 17(4): 1363.
  34. Jaszczak-Wilke, E.; Polkowska; Ż.; Koprowski, M.; Owsianik, K.; Mitchell, A.E. & Bałczewski, P. 2021. Amygdalin: toxicity, anticancer activity and analytical procedures for its determination in plant seeds. Molecules 26(8): 2253.
  35. Kumar, V.; Roy, S.; Behera, B.K. & Das, B.K. 2022. Heat shock proteins (Hsps) in cellular homeostasis: a promising tool for health management in crustacean aquaculture. Life 12(11): 1777.
  36. Kuroda, N.; Naruse, K.; Shima, A.; Nonaka, M.; Sasaki, M. & Nonaka, M. 2000. Molecular cloning and linkage analysis of complement C3 and C4 genes of the Japanese medaka fish. Immunogenetics 51: 117-128.
  37. Lange, M.D. & Webster, C.D. 2017. The effect of temperature on the mucosal IgM antibody response to DNP-KLH in channel catfish (Ictalurus punctatus). Fish & Shellfish Immunology 70: 493-497.
  38. Li, Z.; Liu, Z.; Wang, Y.N.; Kang, Y.J.; Wang, J.F.; Shi, H.N.; Huang, J.Q. & Jiang, L. 2016. Effects of heat stress on serum cortisol, alkaline phosphatase activity and heat shock protein 40 and 90 β mRNA expression in rainbow trout (Oncorhynchus mykiss). Biologia 71(1): 109-115.
  39. Liu, H.; Fu, Z.; Yu, G.; Ma, Z. & Zong, H. 2022. Effects of acute high-temperature stress on physical responses of yellowfin tuna (Thunnus albacares). Journal of Marine Science and Engineering 10(12): 1857.
  40. Lushchak, V.I. & Bagnyukova, T.V. 2006. Temperature increase results in oxidative stress in goldfish tissues. 1. Indices of oxidative stress. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology 143(1): 30-35.
  41. Madaro, A.; Folkedal, O.; Maiolo, S.; Alvanopoulou, M. & Olsen, R.E. 2018. Effects of acclimation temperature on cortisol and oxygen consumption in Atlantic salmon (Salmo salar) post-smolt exposed to acute stress. Aquaculture 497: 331-335.
  42. Magnadóttir, B.; Jónsdóttir, H.; Helgason, S.; Björnsson, B.; Jørgensen, T.Ø. & Pilström, L. 1999. Humoral immune parameters in Atlantic cod (Gadus morhua L.): I. The effects of environmental temperature. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology122(2): 173-180.
  43. Magnadóttir, B.; Lange, S.; Gudmundsdottir, S.; Bøgwald, J. & Dalmo, R.A. 2005. Ontogeny of humoral immune parameters in fish. Fish & Shellfish Immunology 19(5): 429-439.
  44. Mahmoud, S.; Sabry, A.; Abdelaziz, A. & Shukry, M. 2020. Deleterious impacts of heat stress on steroidogenesis markers, immunity status and ovarian tissue of Nile tilapia (Oreochromis niloticus). Journal of Thermal Biology 91: 102578.
  45. Mariana, S. & Badr, G. 2019. Impact of heat stress on the immune response of fishes. Journal of Survey in Fisheries Sciences: 149-159.
  46. McCormick, S.D. 2011. The hormonal control of osmoregulation in teleost fish. Life Sciences 1: 1466-1473.
  47. Mearns, A.J.; Reish, D.J.; Bissell, M.; Morrison, A.M.; Rempel‐Hester, M.A.; Arthur, C.; Rutherford, N. & Pryor, R. 2018. Effects of pollution on marine organisms. Water Environment Research 90(10): 1206-1300.
  48. Mitchell, D.; Snelling, E.P.; Hetem, R.S.; Maloney, S.K.; Strauss, W.M. & Fuller, A. 2018. Revisiting concepts of thermal physiology: predicting responses of mammals to climate change. Journal of Animal Ecology 87(4): 956-973.
  49. Monnier, N.; Furlan, A.L.; Buchoux, S.; Deleu, M.; Dauchez, M.; Rippa, S. & Sarazin, C. 2019. Exploring the dual interaction of natural rhamnolipids with plant and fungal biomimetic plasma membranes through biophysical studies. International Journal of Molecular Sciences 20(5): 1009.
  50. Moslehi, A.; Farahabadi, M.; Chavoshzadeh, S.A.; Barati, A.; Ababzadeh, S. & Mohammadbeigi, A. 2018. The effect of amygdalin on endoplasmic reticulum (ER) stress induced hepatic steatosis in mice. The Malaysian journal of Medical Sciences 25(1): 16.
  51. Munteanu, I.G. & Apetrei, C. 2021. Analytical methods used in determining antioxidant activity: A review. International Journal of Molecular Sciences 22(7): 3380.
  52. Nakano, T.; Kameda, M.; Shoji, Y.; Hayashi, S.; Yamaguchi, T. & Sato, M. 2014. Effect of severe environmental thermal stress on redox state in salmon. Redox Biology 2: 772-776.
  53. Nash, J.P.; Cuisset, B.D.; Bhattacharyya, S.; Suter, H.C.; Le Menn, F. & Kime, D.E. 2000. An enzyme linked immunosorbant assay (ELISA) for testosterone, estradiol, and 17; 20β-dihydroxy-4-pregenen-3-one using acetylcholinesterase as tracer: application to measurement of diel patterns in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry 22: 355-363.
  54. Neiffer, D.L. & Stamper, M.A. 2009. Fish sedation, anesthesia, analgesia, and euthanasia: considerations, methods, and types of drugs. ILAR Journal 50(4): 343-360.
  55. Norouzi, M.; Pourkazemi, M.; Keyvan, A.; Fatemi, S.M.R. & Kazemi, B. 2008. Population genetic structure of stellate sturgeon (Acipenser stellatus Pallas) in the South Caspian Sea using microsatellite markers. Journal of Fisheries and Aquatic Science 3(3): 158-166.
  56. Parvathy, A.J.; Das, B.C.; Jifiriya, M.J.; Varghese, T.; Pillai, D. & Rejish Kumar, V.J. 2023. Ammonia induced toxico‐physiological responses in fish and management interventions. Reviews in Aquaculture 15(2): 452-479.
  57. Portz, D.E.; Woodley, C.M. & Cech, J.J. 2006. Stress-associated impacts of short-term holding on fishes. Reviews in Fish Biology and Fisheries 16: 125-170.
  58. Prashanth, J. & Manjunatha, H. 2022. Transaminases activity in the hemolymph: Biomarkers determining the thermal stress in the new bivoltine lines of Bombyx mori. Journal of Applied Biology and Biotechnology 11(2): 139-143
  59. Qadir, M. & Fatima, K. 2017. Review on pharmacological activity of amygdalin. Archives in Cancer Research 5: 10-12.
  60. Ramsay, J.M.; Feist, G.W.; Varga, Z.M.; Westerfield, M.; Kent, M.L. & Schreck, C.B. 2006. Whole-body cortisol is an indicator of crowding stress in adult zebrafish, Danio rerio. Aquaculture 258(1-4): 565-574.
  61. Ranjan, A.; Srivastava, P.P.; Jain, K.K. & Muralidhar, P. 2020. Comparative evaluation of metabolic enzymes activities in different tissues of Pangasianodon hypophthalmus (Sauvage; 1878) fingerlings reared at higher temperature. Iranian Journal of Fisheries Sciences 19(2): 893-903.
  62. Rao, J.V. 2006. Biochemical alterations in euryhaline fish, Oreochromis mossambicus exposed to sub-lethal concentrations of an organophosphorus insecticide, monocrotophos. Chemosphere 65(10): 1814-1820.
  63. Raposo, A.; Alturki, H.A.; Alkutbe, R. & Raheem, D. 2023. Eating sturgeon: an endangered delicacy. Sustainability 15(4): 3511.
  64. Sadoul, B. & Geffroy, B. 2019. Measuring cortisol, the major stress hormone in fishes. Journal of Fish Biology 94(4): 540-555.
  65. Salmani, E.; Heidari, B;, Vahdatiraad, L. & Zarei, S. 2024. Protection against thermal stress: the role of tex-OE® in inducing HSP70 and boosting defense mechanisms in Acipenser stellatus fry. Aquaculture International 1-19.
  66. Samanta, P.; Pal, S.; Mukherjee, A.K. & Ghosh, A.R. 2014. Evaluation of metabolic enzymes in response to Excel Mera 71; a glyphosate‐based herbicide, and recovery pattern in freshwater teleostean fishes. BioMedical Research International. 2014(1): 425159.
  67. Santos, M.A. & Pacheco, M. 1996. Anguilla anguillaL. Stress biomarkers recovery in clean water and secondary-treated pulp mill effluent. Ecotoxicology and Environmental Safety 35(1): 96-100.
  68. Saurabh, S. & Sahoo, P.K. 2008. Lysozyme: an important defence molecule of fish innate immune system. Aquaculture Research 39(3): 223-239.
  69. Sesay, D.F.; Habte-Tsion, H.M.; Zhou, Q.; Ren, M.; Xie, J.; Liu, B.; Chen, R. & Pan, L. 2017. The effect of dietary folic acid on biochemical parameters and gene expression of three heat shock proteins (HSPs) of blunt snout bream (Megalobrama amblycephala) fingerling under acute high temperature stress. Fish Physiology and Biochemistry 43: 923-940.
  70. Sheikhzadeh, N.; Pashaki, A.K.; Nofouzi, K.; Heidarieh, M. & Tayefi-Nasrabadi, H. 2012. Effects of dietary Ergosan on cutaneous mucosal immune response in rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology 32(3): 407-410.
  71. Shi, L.; Zhao, Y.; Zhou, J.; Zhai, H.; Wei, L.; Liu, Z.; Wang, W.; Jiang, Z.; Ren, T. & Han, Y. 2020. Dietary taurine impacts the growth, amino acid profile and resistance against heat stress of tiger puffer (Takifugu rubripes). Aquaculture Nutrition 26(5): 1691-1701.
  72. Shirmohammadi, M.; Salamat, N.; Ronagh, M.T.; Movahedinia, A. & Hamidian, G. 2018. Using cell apoptosis, micronuclei and immune alternations as biomarkers of phenanthrene exposure in yellowfin seabream (Acanthopagrus latus). Fish & Shellfish Immunology 72: 37-47.
  73. Simide, R.; Gaillard, S. & Richard, S. 2018. The blood indicators of Siberian sturgeon welfare. The Siberian Sturgeon (Acipenser baerii, Brandt; 1869) Volume 2-Farming : 451-477.
  74. Song, Z. & Xu, X.; 2014. Advanced research on anti-tumor effects of amygdalin. Journal of Cancer Research and Therapeutics 10 (Suppl 1): C3-C7.
  75. Sun, B.; Sun, B.; Zhang, B. & Sun, L.; 2022. Temperature induces metabolic reprogramming in fish during bacterial infection. Frontiers in Immunology 13: 1010948.
  76. Vahdatiraad, L.; Heidari, B.; Zarei, S.; Sohrabi, T. & Ghafouri, H. 2024. Potential of heat shock protein inducers in mitigating benzo [α] pyrene toxicity in stellate sturgeon fingerlings (Acipenser stellatus): Assessing the effects on CYP450 and AChE activity, HSP70 expression, antioxidant levels, cortisol, and immunological responses. Journal of the World Aquaculture Society, 55(6): e13092.
  77. Vecsei, P.; Peterson, D.; Suciu, R. & Artyukhin, E. 2007. Threatened fishes of the world, Acipenser stellatus, Pallas; 1771 (Acipenseridae). Environmental Biology of Fishes 78(3): 211-212.
  78. Volkoff, H. & Rønnestad, I. 2020. Effects of temperature on feeding and digestive processes in fish. Temperature 7(4): 307-320.
  79. Wang, B.; Ma, G.; Liu, Y.; Wang, Y.; Du, X.; Shi, Q. & Mao, H. 2021. Effects of different temperatures on the antibacterial, immune and growth performance of crucian carp epidermal mucus. Fishes 6(4): 66.
  80. Wang, Y.; Jia, Q.; Zhang, Y.; Wei, J. & Liu, P. 2020. Amygdalin attenuates atherosclerosis and plays an anti-inflammatory role in ApoE knock-out mice and bone marrow-derived macrophages. Frontiers in Pharmacology 11: 590929.
  81. Xu, L.; Wang, Y.Y.; Huang, J.; Chen, C.Y.; Wang, Z.X. & Xie, H. 2020. Silver nanoparticles: Synthesis, medical applications and biosafety. Theranostics 10(20): 8996.
  82. Yousefi, M.; Adineh, H.; Reverter, M.; Hamidi, M.K.; Vatnikov, Y.A.; Kulikov, E.V.; Hoseinifar, S.H. & Van Doan, H. 2021. Protective effects of black seed (Nigella sativa) diet supplementation in common carp (Cyprinus carpio) against immune depression, oxidative stress and metabolism dysfunction induced by glyphosate. Fish & Shellfish Immunology 109: 12-19.
  83. Zarei, S.; Ghafouri, H.; Vahdatiraad, L.; Heidari, B. & Sohrabi, T. 2024. Enhancing resistance and cell survival in Acipenser ruthenus liver, gill, and kidney cells: The potential of heat shock protein inducers against PAH-benzo [a] pyrene stress. Environmental Science and Pollution Research 31(6): 9445-9460.
  84. Zhang, C.; Zhang, D.; Wang, Y.; Zhang, L.; Qi, S.; Fang, Q.; Xu, Y.; Chen, J.; Cheng, X.; Liu, P. & Wang, C. 2022. Pharmacokinetics and anti-liver fibrosis characteristics of amygdalin: key role of the deglycosylated metabolite prunasin. Phytomedicine 99: 154018.
  85. Zhang, C.N.; Tian, H.Y.; Li, X.F.; Zhu, J.; Cai, D.S.; Xu, C.; Wang, F.; Zhang, D.D. & Liu, W.B. 2014. The effects of fructooligosaccharide on the immune response, antioxidant capability and HSP70 and HSP90 expressions in blunt snout bream (Megalobrama amblycephala Yih) under high heat stress. Aquaculture 433: 458-466.