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Abstract

Abstract
Major plantain (Plantago major) is belonged to Plantaginaceae family, traditionally it was used commonly in folk medicine for skin poultices on wounds, sores, or insect stings. This study was investigated the effect of different levels of major plantain extraction on hematological, biochemical and immunological indices in common carp (Cyprinus carpio). Common carp fingerlings (N=240, 18.69±2.09g) were randomly distributed in 12 tanks (3 treatments in 3 replicates). The fingerlings were fed with 0 (as control), 0.1, 0.5 and 1% major plantain extract for 45 days. At the end of the experiment, blood samples were taken from the caudal vein, and some hematological (RBC, HCT, Hb, MCV, MCH, MCHC, WBC, Lymphocyte, Monocyte, Neutrophil) factors, serum biochemical (Glucose, AST, ALT, ALP) and immunological (Total Ig, Globulin, Albumin, Total protein) indices-indices were evaluated. There were no significant differences in RBC, HCT, MCV, ALP, AST, ALT, Total protein, Albumin and total Immunoglobulin concentrations and also Monocytes, lymphocytes and neutrophils between treatments fed different levels of major plantain extract and the control group (P>0.05). Hb, MCH, MCHC, globulin levels in treatment fed with 0.1% major plantain extract was remarkably higher than other groups (P<0.05). Glucose level in treatments fed with 0.1 and 1% major plantain extract were not remarkably different from each other (P>0.05), but were significantly higher than two other groups (P>0.05). Finally, due to positive effect of major plantain extract at the level of 0.1% on some hematological indices-indices and the immune responses in common carp, it can be suggested to use major plantain extract to promote immune system in common carp.

Keywords

Major plantain Innate immune Biochemical contents Extraction Common carp

Article Details

How to Cite
WAHIDI, A. B., ZAHID, N., MANSOOR, M. A., PAKNEJAD, H., SUDAGAR, M., JAFAR, A., & SHABANY, A. (2024). Dietary Effects of major plantain (<i>Plantago major</i> L.) extraction on hematological, biochemical indices-indices and innate immune system in Common carp (<i>Cyprinus carpio</i>) fingerlings. Iranian Journal of Ichthyology, 11(3), 174–183. https://doi.org/10.22034/iji.v11i3.1028

References

  1. Ahmad, M.S.; Ahmad, M.U. & Osman, S.M. 1980. A new hydroxyolefinic acid from Plantago major seed oil. Phytochemistry 19(10): 2137-2139.
  2. Anushiravani, M.: Azad, F.J.: Taghipour, A.: Mirsadraee, M.: Afshari, J.T.: Salari, R.: Najafzadeh, M.J.: Khoshkhui, M; Engel, R.M. & Farshchi, M.K. 2020. The effect of Plantago major seed and almond gum on refractory asthma: A proof-of-concept study. Journal of Herbal Medicine 19: 100297.
  3. Adel, M.; Dawood, M.A.; Shafiei, S.; Sakhaie, F. & Shekarabi, S.P.H. 2020. Dietary Polygonum minus extract ameliorated the growth performance, humoral immune indices, immune-related gene expression and resistance against Yersinia ruckeri in rainbow trout (Oncorhynchus mykiss). Aquaculture 519: 734738.
  4. Adel, M.; Pourgholam, R.; Zorriehzahra, J. & Ghiasi, M. 2016. Hemato–Immunological and biochemical indices, skin antibacterial activity, and survival in rainbow trout (Oncorhynchus mykiss) following the diet supplemented with Mentha piperita against Yersinia ruckeri. Fish & Shellfish Immunology 55: 267-273.
  5. Alagawany, M.; Farag, M.R.; Salah, A.S. & Mahmoud, M.A. 2020. The role of oregano herb and its derivatives as immunomodulators in fish. Reviews in Aquaculture 12(4): 2481-2492.
  6. Awad, E. & Awaad, A. 2017. Role of medicinal plants on growth performance and immune status in fish. Fish & Shellfish Immunology 67: 40-54.
  7. Binaii, M.; Ghiasi, M.; Farabi, S.M.V.; Pourgholam, R.; Fazli, H.; Safari, R.; Alavi, S.E.; Taghavi, M.J. & Bankehsaz, Z. 2014. Biochemical and hemato-immunological indices in juvenile beluga (Huso huso) following the diet supplemented with nettle (Urtica dioica). Fish & Shellfish Immunology 36(1): 46-51.
  8. Bilen, S.; Filogh, A.M.; Ali, A.B.; Kenanoğlu, O.N. & Zoral, M.A. 2020. Effect of common mallow (Malva sylvestris) dietary supplementation on growth performance, digestive enzyme activities, haemotological and immune responses of common carp (Cyprinus carpio). Aquaculture International 28(1): 73-84.
  9. Bilen, S.; Altief, T.A.S.; Özdemir, K.Y.; Salem, M.O.A.; Terzi, E. & Güney, K. 2020. Effect of lemon balm (Melissa officinalis) extract on growth performance, digestive and antioxidant enzyme activities, and immune responses in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry 46(1): 471-481.
  10. Blaxhall, P.C. & Daisley, K.W. 1973. Routine haematological methods for use with fish blood. Journal of fish biology, 5(6), pp.771-781.
  11. Behbahani, B.A.; Yazdi, F.T.; Shahidi, F.; Hesarinejad, M.A.; Mortazavi, S.A. & Mohebbi, M. 2017. Plantago major seed mucilage: Optimization of extraction and some physicochemical and rheological aspects. Carbohydrate Polymers 155(): 68-77.
  12. Bao, L.; Chen, Y.; Li, H.; Zhang, J.; Wu, P.; Ye, K.; Ai, H. & Chu, W. 2019. Dietary Ginkgo biloba leaf extract alters immune-related gene expression and disease resistance to Aeromonas hydrophila in common carp Cyprinus carpio. Fish & Shellfish Immunology 94:810-818.
  13. Das, R.; Raman, R.P.; Saha, H. & Singh, R. 2015. Effect of Ocimum sanctum Linn. (Tulsi) extract on the immunity and survival of Labeo rohita (Hamilton) infected with Aeromonas hydrophila. Aquaculture Research 46(5): 1111-1121.
  14. Drabkin, D.L. & Austin, J.H. 1932. Spectrophotometric studies: I. Spectrophotometric constants for common hemoglobin derivatives in human, dog, and rabbit blood. Journal of Biological Chemistry 98(2): 719-733.
  15. Elbesthi, R.T.A.; Özdemir, K.Y.; Taştan, Y.; Bilen, S. & Sönmez, A.Y. 2020. Effects of ribwort plantain (Plantago lanceolata) extract on blood indices, immune response, antioxidant enzyme activities, and growth performance in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry 46(4): 1295-1307.
  16. Fazio, F. 2019. Fish hematology analysis as an important tool of aquaculture: a review. Aquaculture 500: 237-242.
  17. FAO. 2006. Yearbooks of Fishery Statistics: Summary Tables.
  18. Feldman, M.S. 2000. Organizational routines as a source of continuous change. Organization Science 11(6): 611-629.
  19. Fu, Y.S.; Lue, S.I.; Lin, S.Y.; Luo, C.L.; Chou, C.C. & Weng, C.F. 2019. Plantago asiatica Seed Extracts Alleviated Blood Pressure in Phase I–Spontaneous Hypertension Rats. Molecules 24(9): 1734.
  20. Giri, S.S.; Sukumaran, V. & Park, S.C. 2019. Effects of bioactive substance from turmeric on growth, skin mucosal immunity and antioxidant factors in common carp, Cyprinus carpio. Fish & Shellfish Immunology 92: 612-620.
  21. Heydarnejad, M.S. 2012. Survival and growth of common carp (Cyprinus carpio L.) exposed to different water pH levels. Turkish Journal of Veterinary & Animal Sciences 36(3): 245-249.
  22. Hoseinifar, S.H.; Zoheiri, F. & Caipang, C.M. 2016. Dietary sodium propionate improved performance, mucosal and humoral immune responses in Caspian white fish (Rutilus frisii kutum) fry. Fish & Shellfish Immunology 55: 23-528.
  23. Hoseinifar, S.H.; Zou, H.K.; Van Doan, H.; Kolangi Miandare, H. & Hoseini, S.M. 2018. Evaluation of some intestinal cytokines genes expression and serum innate immune indices in common carp (Cyprinus carpio) fed dietary loquat (Eriobotrya japonica) leaf extract. Aquaculture Research 49(1): 120-127.
  24. Kesbiç, O.S., 2019. Effects of juniper berry oil on growth performance and blood indices in common carp (Cyprinus carpio). Aquaculture Research 50(1): 342-349.
  25. Mohamed, I.K.; Osama, M.A.; Samiha, M. & Zahrat, E.M.2011. Biochemical studies on Plantago major L. and Cyamopsis tetragonoloba L. International Journal of Biodiversity and Conservation 3: 83-91.
  26. Kurt, B.; Bilge, N.; Sözmen, M.; Aydın, U.; Önyay, T. & Özaydın, İ. 2018. Effects of Plantago lanceolata L. extract on full-thickness excisional wound healing in a mouse model. Biotechnic & Histochemistry 93(4): 249-257.
  27. Latif, M.; Faheem, M.; Hoseinifar, S.H. & Van Doan, H. 2020. Dietary black seed effects on growth performance, proximate composition, antioxidant and histo-biochemical indices of a culturable fish, rohu (Labeo rohita). Animals 11(1): 48.
  28. Lee, D.H.; Lim, S.R.; Ra, C.S. & Kim, J.D. 2012. Effects of dietary garlic extracts on whole body amino acid and fatty acid composition, muscle free amino acid profiles and blood plasma changes in juvenile sterlet sturgeon, Acipenser ruthenus. Asian-Australasian Journal of Animal Sciences 25(10): 1419.
  29. Masamha, B.; Gadzirayi, C.T. & Mukutirwa, I. 2010. Efficacy of Allium sativum (Garlic) in Controlling Nematode Parasites in Sheep. International Journal of Applied Research in Veterinary Medicine 8(3).
  30. Mohammadi, G.; Rashidian, G.; Hoseinifar, S.H.; Naserabad, S.S. & Van Doan, H. 2020. Ginger (Zingiber officinale) extract affects growth performance, body composition, haematology, serum and mucosal immune indices in common carp (Cyprinus carpio). Fish & Shellfish Immunology 99: 267-273.
  31. Mohammadi, M.; Soltani, M.; Siahpoosh, A.; Hosseini Shekarabi, S.P.; Shamsaie Mehrgan, M. & Lymbery, A. 2018. Effect of date palm (Phoenix dactylifera) seed extract as a dietary supplementation on growth performance immunological haematological biochemical indices of common carp. Aquaculture Research 49(8): 2903-2912.
  32. Nhu, T.Q.; Hang, B.T.B.; Hue, B.T.B.; Quetin-Leclercq, J.; Scippo, M.L.; Phuong, N.T. & Kestemont, P. 2019. Plant extract-based diets differently modulate immune responses and resistance to bacterial infection in striped catfish (Pangasianodon hypophthalmus). Fish & Shellfish Immunology 92: 913-924.
  33. Osman, A.G.; Koutb, M. & Sayed, A.E.D.H. 2010. Use of hematological indices to assess the efficiency of quince (Cydonia oblonga Miller) leaf extract in alleviation of the effect of ultraviolet–A radiation on African catfish Clarias gariepinus (Burchell, 1822). Journal of Photochemistry and Photobiology B: Biology 99(1): 1-8.
  34. Pauly, D. & Zeller, D. 2017. Comments on FAOs state of world fisheries and aquaculture (SOFIA 2016). Marine Policy 77: 176-181.
  35. Rajabiesterabadi, H.; Yousefi, M. & Hoseini, S.M. 2020. Enhanced haematological and immune responses in common carp Cyprinus carpio fed with olive leaf extract‐supplemented diets and subjected to ambient ammonia. Aquaculture Nutrition 26(3): 763-771.
  36. Rashidian, G.; Bahrami Gorji, S.; Farsani, M.N.; Prokić, M.D. & Faggio, C. 2020. The oak (Quercus brantii) acorn as a growth promotor for rainbow trout (Oncorhynchus mykiss): growth performance, body composition, liver enzymes activity and blood biochemical indices. Natural Product Research 34(17): pp.2413-2423.
  37. Reina, E.; Al-Shibani, N.; Allam, E.; Gregson, K.S.; Kowolik, M. & Windsor, L.J. 2013. The effects of Plantago major on the activation of the neutrophil respiratory burst. Journal of Traditional and Complementary Medicine 3(4): 268-272.
  38. Roohi, Z.; Imanpoor, M.R.; Jafari, V. & Taghizadeh, V. 2017. The use of fenugreek seed meal in fish diets: growth performance, haematological and biochemical indices, survival and stress resistance of common carp (Cyprinus carpio L.). Aquaculture Research 48(3): 1209-1215.
  39. Rao, Y.V.; Romesh, M.; Singh, A. & Chakrabarti, R. 2004. Potentiation of antibody production in Indian major carp Labeo rohita, rohu, by Achyranthes aspera as a herbal feed ingredient. Aquaculture 238(1-4): 67-73.
  40. Safari, R.; Hoseinifar, S.H.; Nejadmoghadam, S. & Jafar, A., 2016. Transciptomic study of mucosal immune, antioxidant and growth related genes and non-specific immune response of common carp (Cyprinus carpio) fed dietary Ferula (Ferula assafoetida). Fish & Shellfish Immunology 55: 242-248.
  41. Tacon, A.G. 2020. Trends in global aquaculture and aquafeed production: 2000–2017. Reviews in Fisheries Science & Aquaculture 28(1): 43-56.
  42. Alizadeh Doughikollaee, E.; Alizadeh Doghikollaee, E.; Ebrahimi, E. & Zare, P. 2011. Effect of garlic essential oil as an immunostimulant on hematological indices of juvenil beluga (Huso huso). Journal of Veterinary Research 66(3): 209-216.
  43. Temür, C. & Sema, U.S.L.U. 2019. Effects of Plantain (Plantago lanceolata) Containing Diets of Quails on Growth Performance, Carcass Characteristic, Some Blood indices and Mast Cell Numbers. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 29(1): 114-120.
  44. Thompson, P.M.; Tollit, D.J.; Corpe, H.M.; Reid, R.J. & Ross, H.M. 1997. Changes in haematological indices in relation to prey switching in a wild population of harbour seals. Functional Ecology 11(6): 743-750.
  45. Vilizzi, L. & Walker, K. 1998. Age and growth of carp, Cyprinus carpio L. (Cyprinidae), in the River Murray, Australia: validation, consistency of age interpretation and growth models. Environmental Biology of Fishes.
  46. Yeganeh, S.; Teimouri, M. & Amirkolaie, A.K. 2015. Dietary effects of Spirulina platensis on hematological and serum biochemical indices of rainbow trout (Oncorhynchus mykiss). Research in Veterinary Science 101: 84-88.
  47. Yousefi, M.; Hoseini, S.M.; Vatnikov, Y.A.; Kulikov, E.V. & Drukovsky, S.G. 2019. Rosemary leaf powder improved growth performance, immune and antioxidant indices, and crowding stress responses in common carp (Cyprinus carpio) fingerlings. Aquaculture 505: 473-480.
  48. Yousefi, M.; Ghafarifarsani, H.; Hoseinifar, S.H.; Rashidian, G. & Van Doan, H. 2021. Effects of dietary marjoram, Origanum majorana extract on growth performance, hematological, antioxidant, humoral and mucosal immune responses, and resistance of common carp, Cyprinus carpio against Aeromonas hydrophila. Fish & Shellfish Immunology 108: 127-133.
  49. Zemheri-Navruz, F.; Acar, Ü. & Yılmaz, S. 2019. Dietary supplementation of olive leaf extract increases haematological, serum biochemical indices and immune related genes expression level in common carp (Cyprinus carpio) juveniles. Fish & Shellfish Immunology 89: 672-676.
  50. Zakariaee, H.; Sudagar, M.; Hosseini, S.S.; Paknejad, H. & Baruah, K. 2021, In vitro Selection of Synbiotics and in vivo Investigation of Growth Indices, Reproduction Performance, Survival, and Ovarian Cyp19α Gene Expression in Zebrafish Danio rerio, Frontiers in Microbiology 12: 758758.