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Abstract

Finding affordable protein-rich replacements for fishmeal in fish feeds is important for sustainable aquaculture. Lemna minor is emerging as a promising high-protein yet low-cost alternative to traditional fishmeal. Heteropneustes fossilis is a valuable and highly nutritious food fish, but its production in India is low due to the high cost of feed production. In this study, the potential of incorporating L. minor into the diet of H. fossilis was assessed for its impact on growth, digestive enzyme activity, biochemical parameters, and carcass composition. Five isonitrogenous diets LM0 (0%), LM5 (5%), LM10 (10%), LM15 (15%) and LM20 (20%) with varying percentage inclusion of L. minor were fed to H. fossilis fries (0.51±0.01g, 4.1±0.03cm) for 60 days. LM15 diet-fed fish showed better Feed Conversion Ratio (0.93±0.05), Specific Growth Rate (2.60±0.06% day-1), and Protein Efficiency Ratio (2.68±0.14) among all the groups. The inclusion of L. minor increased amylase, lipase, and pepsin activities, whereas chymotrypsin, trypsin and, total protease activities did not differ significantly (P>0.05). Arginine, histidine, methionine, valine and polyunsaturated fatty acids were significantly (P<0.05) elevated in LM15 diets fed fish. Biochemical parameters (thiobarbituric acid reactive substances, superoxide dismutase, and aminotranferases) showed no adverse effect of L. minor on the fish. Our results indicated that L. minor can be added to the fish diet up to 15% for optimum growth without adversely affecting fish health. The results of this study may be useful for the development of a cost-efficient and sustainable plant-based nutrient-rich feed for fish using freely available local resources.

Keywords

Aquafeed Duckweed plant protein stinging catfish Sustainable feed

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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Author Biography

Bronson Kumar KHANGEMBAM, Department of Zoology, Bodoland University, Kokrajhar, BTR, Assam, India.

 

 

How to Cite
MUCHAHARY, S., & KHANGEMBAM, B. K. (2024). Evaluation of dietary <i>Lemna minor</i> supplementation on growth, digestive enzyme activity, and carcass composition of <i>Heteropneustes fossilis</i> (Bloch, 1794). Iranian Journal of Ichthyology, 11(1), 55–68. https://doi.org/10.22034/iji.v11i1.1012
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