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Most pathogenic bacteria have the acquisition of antibiotic resistance. Therefore, continuous research to find a new antibiotic against pathogens is an urgent task to avoid the rapid spreading of diseases. The direction is to create nanomaterials instead of common antibiotics to inhibition some bacteria high effectively. The purpose of this study was to functionalize the gold nanoparticles (AuNPs) with nisin (N) and applied to a group of pathogenic bacteria to evaluate the effectiveness of them in killing the isolated bacteria, including Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Bacillus subtilis, Staphylococcus aureus.  The TEM study revealed the grain size of AuNPs ranging 1-14nm, whereas the grain size of AuNPs-N ranging 1-120nm. Also, we were measured the XRD of AuNPs and AuNPs-N to find out their structural properties. In addition to the evaluation of biologically active substances by FTIR system. The results through the biological activity showed that the nisin functionalized gold nanoparticles (AuNPs-N) exhibited perfect inhibitory activity against all the isolated bacteria.


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AL-HADEDEE, L. T., SAADOUN, H., & AL-MASHHADANI, W. F. (2021). Preparation of Nanogold and binding of nisin to increase its effectiveness in inhibiting the positive and negative bacteria. Iranian Journal of Ichthyology, 8, 290–299.


    Al-hadede, L.T. & Hassan, M.I. 2020. Silver Nanoparticles Synthisis by Green Method and Loading of the Enterosein to Study Its Antimicrobial Inhibition. In IOP Conference Series: Materials Science and Engineering 928(7): 072078.
    AL-Hadedee, L.T.H.; Hasan, S.K.; Mousa, E.F. & Ahmed, W. 2019. Effect of magnetic field treatment on the milk characteristics with the presence of Lactobacillus plantarum and Lactobacillus rhamnosus GG bacteria. Plant Archives 19(1): 275-280.
    Bhat, K.U. & Vidya, S.M. 2017. Nisin gold nanoparticles assemble as potent antimicrobial agent against Enterococcus faecalis and Staphylococcus aureus clinical isolates. Journal of Drug Delivery Science and Technology 37: 20-27.
    Caswell, D.S. & Spiro, T.G. 1987. Proline signals in ultraviolet resonance Raman spectra of proteins: cis-trans isomerism in polyproline and ribonuclease A. Journal of the American Chemical Society 109(9): 2796-2800.
    Chambers, H.F. & DeLeo, F.R. 2009. Waves of resistance: Staphylococcus aureus in the antibiotic era. Nature Reviews Microbiology 7(9): 629-641.
    Chen, H.; Dorrigan, A.; Saad, S.; Hare, D.J.; Cortie, M.B. & Valenzuela, S.M. 2013. In vivo study of spherical gold nanoparticles: inflammatory effects and distribution in mice. PloS one 8(2): e58208.
    Jena, B.K. & Raj, C.R. 2007. Synthesis of flower-like gold nanoparticles and their electrocatalytic activity towards the oxidation of methanol and the reduction of oxygen. Langmuir 23(7): 4064-4070.
    Lowy, F.D. 2003. Antimicrobial resistance: the example of Staphylococcus aureus. The Journal of Clinical Investigation 111(9): 1265-1273.
    Malugin, A. & Ghandehari, H. 2010. Cellular uptake and toxicity of gold nanoparticles in prostate cancer cells: a comparative study of rods and spheres. Journal of Applied Toxicology: An International Journal 30(3): 212-217.
    Saha, B.; Bhattacharya, J.; Mukherjee, A.; Ghosh, A.; Santra, C.; Dasgupta, A.K. & Karmakar, P. 2007. In vitro structural and functional evaluation of gold nanoparticles conjugated antibiotics. Nanoscale Research Letters 2(12): 614-622.
    Sau, T.K. & Murphy, C.J. 2004. Seeded high yield synthesis of short Au nanorods in aqueous solution. Langmuir 20(15): 6414-6420.
    Wang, A.J.; Li, Y.F.; Wen, M.; Yang, G.; Feng, J.J.; Yang, J. & Wang, H.Y. 2012. Melamine assisted one-pot synthesis of Au nanoflowers and their catalytic activity towards p-nitrophenol. New Journal of Chemistry 36(11): 2286-2291.
    Wu, H.Y.; Liu, M. & Huang, M.H. 2006. Direct synthesis of branched gold nanocrystals and their transformation into spherical nanoparticles. The Journal of Physical Chemistry B 110(39): 19291-19294.
    Xu, J.; Li, S.; Weng, J.; Wang, X.; Zhou, Z.; Yang, K. & Zhang, Q. 2008. Hydrothermal syntheses of gold nanocrystals: from icosahedral to its truncated form. Advanced Functional Materials 18(2): 277-284.
    Younes, M.; Aggett, P.; Aguilar, F.; Crebelli, R.;, Dusemund, B. & Gott, D. 2017. Safety of nisin (E 234) as a food additive in the light of new toxicological data and the proposed extension of use. EFSA Journal 15(12): e05063.
    Zhang, H.; Nie, P.; Xia, Z.; Feng, X.; Liu, X. & He, Y. 2020. Rapid Quantitative Detection of Deltamethrin in Corydalis yanhusuo by SERS Coupled with Multi-Walled Carbon Nanotubes. Molecules 25(18): 4081.