Development of liver and pancreas in Acipenser persicus (Borodin, 1897): A histological study

Mohsen ALI, Amin GHOLAMHOSSEINI, Mahdi BANAEE, Peyman NAKHAEE

Abstract

In the present study, different stages of the ontogeny of the liver and pancreas in the Acipenser persicus were illustrated from hatching until 36 days post hatching (dph) by histological techniques. The histological sections (sagittal plane) were made by a microtome at a thickness of 5 µm and stained with Hematoxylin and Eosin, Periodic Acid-Schiff and Masson's trichrome. Based on the results, in 2 dph, liver stroma was formed and stellate hepatocytes included numerous vacuoles with nuclei situated parametrically. On 10 dph, the pancreas was observed between the liver and anterior part of the intestine for the first time. Exocrine pancreatic cells were organized in acini. At 18 dph, the central veins and hepatic sinusoids were remarkably apparent in the middle of the liver parenchyma. The pancreas grew as a basophilic organ and it was situated in the posterior intestine on the 22-dph, bile duct, portal vein and hepatic artery was acquiring their structure in connective tissue. On day 36, the liver was characterized by an enlarged and organized form. Formation of the hepatopancreatic tissue is completed within 16 to 36 days after hatching.

Keywords

Persian sturgeon, Liver, Pancreas, Ontogeny, Histology.

Full Text:

PDF

References

Abdali, H. & Eagderi, S. 2015. Ontogeny of gill structure in Sterlet, Acipenser ruthenus (Linnaeus, 1758). Iranian Journal of Ichthyology 2(2): 87-92.

Abol-Munafi, A.B.; Liem, P.T.; Van, M.V.; Ambak, M.A.; Effendy, A.W. M. & Soh, M.A. 2006. Histological ontogeny of the digestive system. Journal of sustainability Science and Management 1(2): 79-86.

Akiyoshi, H. & Inoue, A. 2004. Comparative histological study of teleost livers in relation to phylogeny. Zoological science 21(8): 841-850.

Alboghobeish, N. & Khaksar-Mahabady, M. 2005. Histological study of liver and pancreas in Cetonopharingodon idella. Journal of Veterinary Medical Education 11: 25-34.

Babaei, S.S.; Abedian Kenari, A.M.; Nazari, R. M. & Gisbert, E. 2011. Developmental changes of digestive enzymes in Persian sturgeon (Acipenser persicus) during larval ontogeny. Aquaculture 318: 138-144.

Banaee, M. & Naderi, M. 2014. The Reproductive Biology of Shirbot (Barbus grypus Heckel, 1843) in the Maroon River, Iran. International Journal of Aquatic Biology 2(1): 43-52.

Batebi Navaei, M.; Mojazi Amiri, B.; Mohammad Nazari, R.; Nematolahi, M.A. & Karaminasab, M. 2015. A histochemical of developing digestive tract of Persian sturgeon (A. persicus) from hatching to fingerling. Journal of Fisheries 6802: 187-197.

Billard, R. & Lecointre, G. 2001. Biology and conservation of sturgeon and paddlefish. Reviews in Fish Biology and Fisheries 10: 355-392.

Boglione, C.; Bronzi, P.; Cataldi, E.; Serra, S.; Gagliardi, F. & Cataudella, S. 1999. Aspects of early development in the Adriatic sturgeon Acipenser naccarii. Journal of Applied Ichthyology 15: 207-213.

Buddington, R.K. & Christofferson, P. 1985. Digestible and feeding characteristics of the Chondrosteans. In: F.P. Binkowski & S.I. Doroshov (Eds.). North American Sturgeons: Biology and Aquaculture Potential, DR W. Junk Publishers, Hingham, MA, USA. pp: 31-41.

Buddington, R.K. & Doroshov, S.I. 1986. Development of digestive secretions in white sturgeon juveniles (Acipenser transmontanus). Comparative Biochemistry and Physiology 83: 233-238.

Çinar, K. & Şenol N. 2005. Development of the liver and pancreas in the rainbow trout (Oncorhynchus mykiss). Eğirdir Su Ürünleri Fakültesi Dergisi, 2: 1-6.

Eagderi S.; Poorbagher H.; Moshayedi F. & Hosseini S.V. 2017. Morphological development and allometric growth patterns of Acipenser persicus Borodin, 1897 (Actinopterygii, Acipenseridae) during early development. International Journal of Aquatic Biology 5(3): 201-207.

Faustino, F.; Makino, L.C.; Neumann, E. & Nakaghi, L.S.O. 2018. Histological description of the larval development of Brycon gouldingi Lima, 2004 (Teleostei, Characidae). International Journal of Aquatic Biology 6(2): 75-87.

Gawlicka, A.; Hung, S.S.O.; Hinton, D.E. & de Ia Noüe, J. 1995. Histological and histochemical changes in the digestive tract of white sturgeon larvae during ontogeny. Fish Physiology and Biochemistry 14 (5): 357–371.

Ghasemi, N.; Imani, A.; Noori, F. & Shahrooz, R. 2020. Ontogeny of digestive tract of stellate sturgeon (Acipenser stellatus) from hatching to juvenile stage: Digestive enzymes activity, stomach and proximal intestine. Aquaculture 519: 734751

Jafaryan, H.; Akhoundian, M.; Mehrpoosh, M.; Kabir, M.; Hajirezaee, S. & Golpour, A. 2011. Changes of stress indicators during surgery of female Persian sturgeon. Journal of Cell and Animal Biology 5(10): 219-222.

Lipscomb, T.N.; Yanong, R.P.; Ramee, S.W. & DiMaggio, M.A. 2020. Histological, histochemical and biochemical characterization of larval digestive system ontogeny in black tetra Gymnocorymbus ternetzi to inform aquaculture weaning protocols. Aquaculture 520: 734957.

Mani-Ponset, L.; Guyot, E.; Diaz, J.P. & Connes, R. 1996. Utilization of yolk reserves during post-embryonic development in three teleostean species: the sea bream, (Sparus aurata), the sea bass, (Dicentrarchus labrax) and the pike-perch (Stizostedion lucioperca). Marine Biology 126: 539-547.

Ostaszewska, T. 2005. Developmental changes of digestive system structures in pike-perch (sander lucioperca L.). Electronic Journal of Ichthyology 2: 65-78.

Ostaszewska, T.; Kolman, R.; Kamaszewski, M.; Wiszniewski, G.; Adamek, D. & Duda, A. 2011. Morphological changes in digestive tract of Atlantic sturgeon Acipenser oxyrinchus during organogenesis. International Aquatic Research 3: 101-105.

Ozawa, A. & Sakaue, M. 2020. New decolorization method produces more information from tissue sections stained with hematoxylin and eosin stain and masson-trichrome stain. Annals of Anatomy-Anatomischer Anzeiger 227: 151431.

Pieler, T. & Chen, Y. 2006. Forgotten and novel aspects in pancreas development. Biological of the Cell, 98: 79–88.

Pousty, I. & Sedigh-Marvasti, S.A.H. 2000. Atlas of Fish Histology. 2Edn. University of Tehran Press, Tehran, Iran. 328 p.

Sanz, A.; Orente, J.I.L.; Furne, M.; Ostos-Garrido, M.V.; Carmona, R.; Domezain, A. & Hidalgo M.C. 2011. Digestive enzymes during ontogeny of the sturgeon Acipenser naccarii: intestine and pancreas development. Journal of Applied Ichthyology 27: 1139-1146.

Sheybani, M.T. & AdibMoradi, M. 2002. Histological study of the liver and pancreas and their ducts in Acipenser stellatus. Journal of Veterinary Research 57(1):19-23.

Suwanjarat, J.; Saelee, T.; Kiriratnikon, S.; Thongboon, L. & Pongsuwan. K. 2011. The development of the digestive system in Clarias Nieuhofii larvae: histology and histochemical studies. Journal of the Microscopy Society of Thailand 4 (1): 16-19.

Tao, T. & Peng, J. 2009. Liver development in zebrafish (Danio rerio). Journal of Genetics and Genomics 36: 325-334.

Teresa, O. 2005. Developmental changes of digestive system structures in pike-perch (Sander lucioperca L.). Electronic Journal of Ichthyology 2: 65-78.

Wegner, A.; Ostaszewska, T. & Rozek, W. 2009. The ontogenetic development of the digestive tract and accessory glands of sterlet (Acipenser ruthenus L.) larvae during endogenous feeding. Review of Fish Biological Fisheries 19: 431-444.

Yousefian, M. & Najafpour, S.H. 2011. Enrichment of Artemia Using Highly Unsaturated FattyAcid and Vitamin C in Larval Culture of Acipenser persicus. World Applied Sciences Journal 12(8): 1266-1268.

Zambonino-Infante, J.L.; Gisbert, E.; Sarasquete, C.; Navarro, I.; Gutiérrez, J. & Cahu, C.L. 2008. Ontogeny and physiology of the digestive system of marine fish larvae. Feeding and Digestive Functions of Fishes 281-348.

Refbacks

  • There are currently no refbacks.