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Abstract

The objective of this study was to investigate the effects of combination of Dimethoate pesticide and Bio-fertilizer, two frequently used chemical in agriculture, on blood biochemical and physiological parameters of common carp, Cyprinus carpio to understand their potential hazard to freshwater organisms. A total of 270 fish specimens were selected and allocated into nine treatments including: control group, A (0.016 mg/L pesticide), B (0.032 mg/L pesticide), C (0.1 ml/L fertilizer), D (0.2 ml/L fertilizer) and combined treatments: AC, AD, BC, BD, in triplicate. After 14 days of experiment, bloods were sampled from caudal vein. The blood biochemical enzymes including Acetylcholinesterase (AChE), Alkaline Phosphatase (ALP), Aspartate aminotransferase (AST), Lactate dehydrogenase (LDH), Alanine aminotransferase (ALT) and Creatine phosphokinase (CPK), were measured. AChE showed a decreasing trend in all treatments except in control fish. ALP significantly decreased in groups received higher doses of fertilizer and combined treatment of lower doses of pesticide with both doses of fertilizer (D, AC, AD treatments). AST and LDH did not show any significant differences in all treatments compared to the control group. ALT in combined AD treatment showed a significant increase compared to the control group and other treatments. CPK in B and combined treatments significantly increased in comparison with the control group. The results of this study showed that the effects of combined treatments of dimethoate pesticide and bio-fertilizer were significantly different from those in separate treatments of given chemicals. 

Keywords

Dimethoate pesticide Bio-fertilizer Common carp Biochemical parameters.

Article Details

How to Cite
FAZILAT, N., VAZIRZADEH, A., BANAEE, M., & FARHADI, A. (2017). Separate and combined effects of Dimethoate pesticide and bio-fertilizer on the activity of enzymes involved in anaerobic pathway, neurotransmission and protein metabolism in common carp, Cyprinus carpio (Teleostei: Cyprinidae). Iranian Journal of Ichthyology, 4(4), 352–360. https://doi.org/10.22034/iji.v4i4.241

References

    Agrahari, S.; Pandey, K.C. & Gopal, K. 2007. Biochemical alteration induced by monocrotophos in the blood plasma of fish, Channa punctatus (Bloch). Pesticide Biochemistry and Physiology 88: 268-272.
    Banaee, M.; Haghi, B.N. & Ibrahim, A.T.A. 2014. Sub-lethal toxicity of chlorpyrifos on common carp, Cyprinus carpio (Linnaeus, 1758): Biochemical response. International Journal of Aquatic Biology 1: 281-288.
    Banaee, M.; & Sureda, A. 2011. Effects of long-term silymarin oral supplementation on the blood biochemical profile of rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry 37: 885-896.
    Banaee, M.; Shahafve, S.; Vaziriyan, M.; Taheri, S. & Haghi, B.N. 2016. Effects of sewage effluent on blood biochemical parameters of common Carp (Cyprinus carpio): A case Study of Behbahan, Khuzestan Province. Journal of Chemical Health Risks 6: 161- 173.
    Banaee, M.; Sureda, A.; Zohiery, F.; Hagi, B.N. & Garanzini, D.S. 2014b. Alterations in biochemical parameters of the freshwater fish, Alburnus mossulensis, exposed to sub-lethal concentrations of Fenpropathrin. International Journal of Aquatic Biology 2: 58-68.
    Bernet, D.; Schmidt, H.; Wahli, T. & Burkhardt-Holm, P. 2001. Effluent from a sewage treatment works causes changes in serum chemistry of brown trout (Salmo trutta L.). Ecotoxicology and Environmental Safety 48: 140-147.
    Dey, C. & Saha, S. 2014. A comparative study on the acute toxicity bioassay of dimethoate and lambda-cyhalothrin and effects on thyroid hormones of freshwater teleost fish Labeo rohita (Hamilton). International Journal of Environmental Research 8: 1085-1092.
    Fulton, M.H. & Key, P.B. 2001. Acetylcholinesterase inhibition in estuarine fish and invertebrates as an indicator of organophosphorus insecticide exposure and effects. Environmental Toxicology and Chemistry 20: 37-45.
    Hussain, M.I.; Kumar, B. & Ahmad, M. 2016. Effect of organophosphate insecticide, Dimethoate on physiology of common carp, Catla catla (Hamilton) and Labeo rohita. International Journal of Current Microbiology and Applied Sciences 5: 322-341.
    Kaya, H.; Çelik, E.Ş.; Yılmaz, S.; Tulgar, A.; Akbulut, M. & Demir, N. 2015. Hematological, serum biochemical, and immunological responses in common carp (Cyprinus carpio) exposed to phosalone. Comparative Clinical Pathology 24: 497-507.
    Monteiro, D.A.; De Almeida, J.A.; Rantin, F.T. & Kalinin, A.L. 2006. Oxidative stress biomarkers in the freshwater characid fish, Brycon cephalus, exposed to organophosphorus insecticide Folisuper 600 (methyl parathion). Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology 143: 141-149.
    Rajendran, K. & Devaraj, P. 2004. Biomass and nutrient distribution and their return of Casuarina equisetifolia inoculated with biofertilizers in farm land. Biomass and Bioenergy 26: 235-249.
    Rao, J.V. 2006. Sublethal effects of an organophosphorus insecticide (RPR-II) on biochemical parameters of tilapia, Oreochromis mossambicus. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmacology 143: 492-498.
    Saha, S. & Kaviraj, A. 2009. Effects of cypermethrin on some biochemical parameters and its amelioration through dietary supplementation of ascorbic acid in freshwater catfish Heteropneustes fossilis. Chemosphere 74: 1254-1259.
    Shakoori, A.R.; Mughal, A.L. & Iqbal, M.J. 1996. Effects of sublethal doses of fenvalerate (a synthetic pyrethroid) administered continuously for four weeks on the blood, liver, and muscles of a freshwater fish, Ctenopharyngodon idella. Bulletin of Environmental Contamination and Toxicology, 57(3): 487-494.
    Singh, R.N. 2009. Acute toxicity and behavioral responses of common carp Cyprinus carpio (Linn.) to an organophosphate (Dimethoate). World Journal of Zoology 4: 70-75.
    Singh, R.N. 2013. Acute toxicity of an organophosphate, dimethoate to an air breathing fish, Colisa fasciatus (Bl. & Schn.). Indian Journal of Scientific Research 4: 97.
    Vani, T.; Saharan, N.; Mukherjee, S.; Ranjan, R.; Kumar, R. & Brahmchari, R. 2011. Deltamethrin induced alterations of hematological and biochemical parameters in fingerlings of Catla catla (Ham.) and their amelioration by dietary supplement of vitamin C. Pesticide Biochemistry and Physiology 101: 16-20.
    Velisek, J.; Svobodova, Z. & Machova, J. 2009. Effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). Fish Physiology and Biochemistry 35: 583-590.
    Wu, S.; Cao, Z.; Li, Z.; Cheung, K. & Wong, M. 2005. Effects of biofertilizer containing N-fixer, P and K solubilizers and AM fungi on maize growth: a greenhouse trial. Geoderma 125: 155-166.