Taxonomic status of the genus Cobitis (Teleostei: Cobitidae) in the Namak Lake basin, Iran



This study was aimed to report the presence of the genus Cobitis in the Karaj River, Namak Lake basin, Iran and clarify its taxonomic status by providing morphological characteristics, mtDNA COI barcode region and its phylogenetic relationship within the members of the genus Cobitis in Iran. The results revealed that morphometric, meristic and molecular (COI) characters of these specimen are largely overlapping or even identical with those of C. faridpaki. Therefore, we conclude that they are a population belonging to C. faridpaki.


Taxonomy, Morphology, COI, Spined loach, Distribution.

Full Text:



Altschul, S.F.; Gish, W.; Miller, W.; Myers, E.W. & Lipman, D.J. 1990. Basic local alignment search tool. Journal of Molecular Biology 215(3): 403-410.

Bohlen, J. & Ráb, P. 2001. Species and hybrid richness in spined loaches of the genus Cobitis (Teleostei: Cobitidae), with a checklist of European forms and suggestions for conservation. Journal of Fish Biology, 59: 75-89.

Carlton, J.T. 2007. The Light and Smith Manual: Intertidal Invertebrates from Central California to Oregon. 4th Revised edition, University of California Press.1140p.

Coad, C. 2017. Iranian Freshwater Fishes. Retrieved from

Eagderi, S.; Jouladeh-Roudbar, A.; Jalili P.; Sayyadzadeh, G. & Esmaeili, H.R. 2017. Taxonomic status of the genus Cobitis Linnaeus, 1758 (Teleostei: Cobitidae) in the southern Caspian Sea basin, Iran with description of a new species. FishTaxa 2(1): 48-61.

Eschmeyer, W.N. & Fong, J.D. 2011. Pisces. In: Zhang, Z.-Q. (Ed.). Animal biodiversity: An outline of higher level classification and survey of taxonomic richness. Zootaxa 3148: 26-38.

Esmaeili, H. R.; Mehraban, H.; Abbasi, K.; Keivany, K. & Coad B. W. 2017. Review and updated checklist of freshwater fishes of Iran: taxonomy, distribution and conservation status. Iranian Journal of Ichthyology v. 4 (Suppl. 1): 1-114.

Jouladeh-Roudbar, A.; Vatandoust, S.; Eagderi, S.; Jafari-Kenari, S. & Mousavi-Sabet, H. 2015. Freshwater fishes of Iran; an updated checklist. AACL Bioflux 8(6): 855-909.

Jouladeh-Roudbar, A.; Eagderi, S.; Sayyadzadeh, G. & Esmaeili, H.R. 2017. Cobitis keyvani, a junior synonym of Cobitis faridpaki (Teleostei: Cobitidae). Zootaxa 4244(1): 118-126.

Hashemzadeh Segherloo, I.; Bernatchez, L.; Golzarianpour, K.; Abdoli, A.; Primmer, C.R. & Bakhtiary, M. 2012. Genetic differentiation between two sympatric morphs of the blind Iran cave barb Iranocypris typhlops. Journal of Fish Biology 81(5): 1747-1753.

Huelsenbeck, J.P. & Rannala, B. 2004. Frequentist properties of Bayesian posterior probabilities of phylogenetic trees under simple and complex substitution models. Systematic Biology 53(6): 904-913.

Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111-120.

Kottelat, M. & Freyhof, J. 2007. Handbook of European Freshwater Fishes. Cornol, Switzerland: 646p.

Mousavi-Sabet, H.; Vasil’eva, E.D.; Vatandoust, S. & Vasil’ev, V.P. 2011. Cobitis faridpaki sp. nova—a new spined loach species (Cobitidae) from the Southern Caspian Sea basin (Iran). Journal of Ichthyology 51(10): 925-931.

Mousavi-Sabet, H.; Vatandoust, S.; Esmaeili, H.R.; Geiger, M.F. & Freyhof, J. 2015. Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae). Zootaxa 3914(5): 558-568.

Nakamura, T. 2003. Meristic and Morphometric Variations in the Fluvial Japanese Charr between River Systems and Among Tributaries of a River System. Environmenta Biology of Fish 66: 133-141.

Nelson, J.S.; Grande, T.C. & Wilson, M.V.H. 2016. Fishes of the World, 5th Edition. John Wiley & Sons, Inc., Hoboken, New Jersey, USA. 752p.

Nei, M. & Kumar, S. 2000. Molecular evolution and phylogenetic. Oxford University Press. 352p.

Posada, D. & Crandall, K.A. 1998. Modeltest: testing the model of DNA substitution. Bioinformatics 14(9): 817-818.

Posada, D. & Crandall, K.A. 2001. Selecting the best-fit model of nucleotide substitution. Systematic Biology 50(4): 580-601.

Rambaut, A. & Drummond, A.J. 2013. Tracer v1. 5 Available from http://beast. bio. ed. ac. uk/Tracer.

Ronquist, F. & Huelsenbeck, J.P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19(12): 1572-1574.

Sawada, Y. 1982. Phylogeny and zoogeography of the superfamily Cobitoidea (Cyprinoidei, Cypriniformes). Memoirs of the Faculty of Fisheries of Hokkaido University 28(2): 65-223.

Tamura, K.; Stecher, G.; Peterson, D.; Filipski, A. & Kumar, S. 2013. MEGA6: Molecular Evolutionary Genetics Analyses version 6.0. Molecular Biology and Evolution 30: 2725-2729.


  • There are currently no refbacks.