Comparative studies on histology and histochemistry of pancreas between Labeo calbasu (Hamilton, 1822) and Mystus gulio (Hamilton, 1822)



The cellular architecture and histochemical nature of the enzyme secretory acinar cells of pancreas have been investigated in two species of fish, Labeo calbasu and Mystus gulio having different feeding habits. Histological analysis illustrates that the exocrine pancreatic tissues are scattered within the hepatic parenchyma as hepatopancreas and spleen as spleenpancreas in L. calbasu. The structural analysis also shows that exocrine as well as endocrine pancreatic tissues are also diffused in the adipose tissues in between the intestinal coiling of L. calbasu. In M. gulio the discrete pancreatic tissue is attached to the outer wall of the stomach and formed cluster of acini interspersed with small areas of islet of Langerhans and blood vessels. The exocrine pancreatic acinar cells are provided with conspicuous nuclei and densely packed acidophilic zymogen granules. The endocrine component of pancreas, islet of Langerhans is encircled by a thin connective tissue and comprised of α, β and δ cells. Different cell types in the islets are differentiated based mainly upon their shape, position, staining intensity and density of the cytoplasmic secretory granules. α cells contain sparse cytoplasm and densely stained nuclei, are generally located to the periphery of the islet Aldehyde fuchsin positive β cells bear spherical nucleus and granular cytoplasm, are in the center of islet of Langerhans contacted with blood vessels. δ cells are few in number with light stained nuclei disperse in the pancreatic islet. Histochemical detection displays that the zymogen granules of exocrine acinar cells showing variegated intensities of tryptophan reaction, the precursor of numerous pancreatic enzymes which probably related to the food and feeding habits of the fishes concerned. The zymogen granules are larger in size and heavily deposited in the acinar cells of hepatopancreas of L. calbasu in comparison to M. gulio may be correlated with the herbivorous feeding habit in respect to secretion of digestive enzymes.


Histoarchitecture, Tryptophan, Pancreas, Structural analysis, Cyprinidae, Bagridae.

Full Text:



Abad, M.E.; Agulleiro, B. & Rombout, J.H. 1986. An immunocytochemical andultrastructural study of the endocrine pancreas of Sparus auratus L. (Teleostei). General and Comparative Endocrinology 64:1-12.

Adams, C.W.M. 1957. p-dimethylaminobenzaldehyde nitrate method for the histochemical demonstration of tryptophan and related compounds. Journal of Clinical Pathology 10: 56-62.

Alboghohobeish, N. & Khaksary Mahabady, M. 2005. Histological study of liver and pancreas in Ctenopharyngodon idella. Journal of School of Veterinary Medicine, Shahid Chamran University, Ahvaz 11: 25-34.

Al-Mahrouki, A.A. & Youson, J.H. 1999. Ultrastructure and immunocytochemistry of the islet organ of Osteoglossomorpha (Teleostei). General and Comparative Endocrinology 116: 409-421.

Beccaria, C.; Diaz, J.P. & Connes, R. 1992. Effects of dietry conditions on the exocrine pancreas of the sea bass, Dicentrarchus labrax L. (Teleostei). Aquaculture 101: 163-176.

Chakrabarti, P. & Ghosh, S.K. 2012. Comparative histophysiology of pancreas in Cirrhinus mrigala (Hamilton, 1822), Oreochromis nilotica (Linnaeus, 1758) and Wallago attu (Bloch & Schneider, 1801). Indian Journal of Fisheries 59: 93-99.

Chakrabarti, P. & Ghosh, S.K. 2015. Comparative histological and histochemical studies on the pancreas of Labeo rohita (Hamilton, 1822), Mystus vittatus (Bloch, 1790) and Notopterus notopterus (Pallas, 1769). International Journal of Aquatic Biology 3: 28-34.

Dwivedi, A.K. & Pandey, D.N. 1982. Observation on the pancreas of a freshwater fish, Mystus vittatus (Bloch). Reading in Zoology 1: 48-49.

Elbal, M.T.; Lozano, M.T. & Agulleiro, B. 1988. The endocrine cells in the gut of Mugil saliens Risso, 1810 (Teleostei): an immunocytochemical and ultrastructural study. General and Comparative Endocrinology 70: 231-246.

Faccioli, C.K.; Chedid, R.A.; Bombonato, M.T.S.; Vicentini, C.A. & Vicentini, I.B.F. 2014. Morphology and histochemistry of the liver of carnivorous fish Hemisorubim platyrhynchos. International Journal of Morphology 32: 715-720.

Field, H.A.; Si Dong, P.D.; Beis, D. & Stainier D.Y.R. 2003. Formation of the digestive system in zebra fish. ii pancreas morphogenesis. Developmental Biology 261: 197-208.

Fortin, J.S.; Santamaria-Bouvier, A.; Lair, S.; Dallaire, A.D. & Benoit-Biancamano, M. 2015. Anatomical and molecular characterization of the endocrine pancreas of ateleostean fish: Atlantic wolfish (Anarhichas lupus). Zoological Studies 54: 21.

Geyer, H.J.; Nel, M.N. & Swanepoel, J.H. 1996. Histology and ultrastructure of the hepatopancreas of the tigerfish, Hydrocynus forskahlii. Journal of Morphology 227: 93-100.

Ghosh, S.K.; Barun, S. & Chakrabarti, P. 2016. A comparative study of the histoarchitecture of endocrine pancreas in Labeo bata (Hamilton, 1822), Sperata aor (Hamilton, 1822) and Chitala chitala (Hamilton, 1822). International Journal of Aquatic Biology 4: 17-24.

Gomori, G. 1941. Observations with differential stains on human islets of Langerhans. American Journal of Pathology 17: 395.

Gonzalez, G.; Crespo, S. & Brusle, J. 1993. Histo–cytological study of the liver of the cabrilla sea bass, Serranus cabrilla (Teleostei, Serranidae) an available model for marine fish experimental studies. Journal of Fish Biology 43: 363-373.

Halami, N.S. 1952. Differentiation of the two types of basophils in the adenohypophysis of the rat and the mouse. Stain Technology 27: 61.

Hossain, M.Y.; Islam, R.; Hossen, M.A.; Rahman, O.; Hossain, M.A.; Islam, M.A. & Alam, M.J. 2015. Threatened fishes of the world: Mystus gulio (Hamilton, 1822) (Siluriformes: Bagridae). Croatian Journal of Fisheries 73: 43-45.

Iaglov, V.V. 1978. Morphology of the endocrine portion of the teleost pancreas. Arkhiv Anatomii, Gistologii I Embriologii 74: 111-115.

Khaksary Mahabady, M.; Morovvati, H.; Arefi, A. & Karamifar, M. 2012. Anatomical and histomorphological study of spleen and pancreas in Berzem (Barbus pectoralis). World Journal of Fish and Marine Sciences 4: 263-267.

Khanna, S.S. 1963. On the pancreas of some freshwater teleosts. Journal of Zoological Society of India 3: 1-19.

Kiernan, J.A. 2008. Histological and histochemical methods: theory and practice. 4th Edition. Scion Publishing, Bloxham, UK.

Klein, C. & Van, N.S. 1978. Use of immunocytochemical staining of somatostatin for correlative light and electron microscopic investigation of D cells in the pancreatic islet of Xiphophorus helleri H. (Teleostei). Cell and Tissue Research 194: 399-404.

Legouis, P. 1873. Recherches sur les tubes de weber et sur le pancréas des poissons osseux. Annales des Sciences Naturelles-Zoologie et Biologie Animale 17: 1-107.

Mallory, F.B. 1936. The aniline blue collagen stain. Stain Technology 11: 101.

Mokhtar, D.M. 2015. Histological, histochemical and ultrastructural characterization of the pancreas of the grass carp (Ctenopharyngodon idella). European Journal of Anatomy 19: 145-153.

Naguib, S.A.; Rizkalla, W. & Abd EI-Ghafar, F.A. 2009. Comparative histological and ultrastructural studies on the liver and pancreas of Schilbe mystus and Labeo niloticus. Egyptian Journal of Aquatic Biology and Fisheries 13: 107-127.

Nejedli, S. & Gajger, I.T. 2013. Hepatopancreas in some sea fish from different species and the structure of the liver in teleost fish, common Pandora, Pagellus erythinus (Linnaeus, 1758) and whiting, Merlangius merlangus euxinus (Nordmann, 1840). Veterinarski Arhiv 83: 441-452.

Pandian, T.J. 1968. Feeding habits of the fish Megalops cyprinoides in the Cooum backwaters, Madras. Journal of the Bombay Natural History Society 65: 569-580.

Petcoff, G.M.; Diaz, A.O.; Escalante, A.H. & Goldenberg, A.L. 2006. Histology of the liver of Oligosarcus jenysii (Ostariophysi, Characidae) from Los Padres Lake, Argentina. Iheringia Série Zoologia 96: 205-208.

Plisetskaya, E.M.; Pollock, H.G.; Rouse, J.B.; Hamilton, J.W.; Kimmel, J.R. & Gorbman, A. 1985. Characterization of coho salmon (Oncorhynchus kisutch) insulin. Regulatory Peptides 11: 105-116.

Plisetskaya, E.M.; Pollock, H.G.; Rouse, J.B.; Hamilton, J.W.; Kimmel, J.R. & Gorbman, A. 1986. Isolation and structures of coho salmon (Oncorhynchus kisutch) glucagon and glucagon-like peptide. Regulatory Peptides 14: 57-67.

Shyamasundari, K.; Raja Kumari, V.J.V. & Hanumantha Rao, K. 2006. Observation on the pancreas of the marine lizard fish Saurida tumbil (Bloch). Journal of Fish Biology 21: 449-454.

Sayrafi, R.; Najafi, G.; Rahmati-holasoo, H.; Hooshyari, A; Akbari, R.; Shokrpoor, S. & Ghadam, M. 2011. Histological study of hepatopancreas in Hi Fin Pangasius (Pangasius sanitwongsei). African Journal of Biotechnology 10: 3463-3466.

Sheybani, M.T.; Adibmoradi, M. 2002. Histological study of the liver and pancreas and their ducts in Acipenser stellatus. Journal of Faculty of Veterinary Medicine, University of Tehran 57: 19-23.

Shrivastava, S. & Chaurasia, R.C. 1976. Pancreas in certain freshwater teleosts. Current Science 45: 144.

Singh, P.R. & Singh, H.R. 2000. Feeding biology of Labeo calbasu (Ham.). Proceedings of the National Academy of Sciences India. Section B, Biological Sciences 70: 179-183.

Sinha, G.M. & Moitra, S.K. 1975. Functional morpho-histology of the alimentary canal of an Indian freshwater major carp Labeo rohita (Hamilton), during its different life-history stages. Anatomische Anzizer 138: 222-239.

Sorokin, A.V.; Petrenko, O.I.; Kavsan, V.M.; Kozlov, Y.I.; Debabov, V.G. & Zlochevskij, M.L. 1982. Nucleotide sequence analysis of the cloned salmon preproinsulin cDNA. Gene 20: 367-376.

Stefan, Y. & Falkmer, S. 1980. Identification of four endocrine cell types in the pancreas of Cottus scorpius (Teleostei) by immunofluorescence and electron microscopy. General and Comparative Endocrinology 42: 171-178.

Suvarna, K.S.; Layton, C. & Bancroft, J.D. 2013. Bancroft’s theory and practice of histological techniques. Elsevier, Churchill Livingstone, Beijing, China.

Talwar, P.K.; Jhingran, A.G. 1991. Inland fishes of India and adjacent countries. Balkema, Rotterdam.

Vicentini, C.A.; Franceschini-Vicentini, I.B.; Bombonato, M.T.S.; Bertolucci, B; Lima, S.G. & Santos, A.S. 2005. Morphological study of the liver in the teleost Orechromis niloticus. International Journal of Morphology 23: 211-216.

Wang, Y.Q.; Plisetskaya, E.; Baskin, D.G. & Gorbman, A. 1986. Immunocytochemical study of the pancreatic islets of the Pacific salmon, Oncorhynchuys kisutch. Zoological Science 3: 123-129.

Yamane, S. 1973. Localization of amylase activity in digestive organs of carp determined by a substrate film method. Bulletin of the Japanese Society of Scientific Fisheries 39: 497-504.

Youson, J.H.; Al-Mahrouki, A.A.; Naumovski, D. & Conlon, J.M. 2001. The endocrine cells in the gastroenteropancreatic system of the Bowfin, Amia calva L.: an immunohistochemical, ultrastructural, and immunocytochemical analysis. Journal of Morphology 250: 208-224.


  • There are currently no refbacks.