Main Article Content
Abstract
The present study is related to the histochemical investigations on the changes in the intensity of reactions of axons, acid and neutral mucopolysaccharides, biologically active macromolecules, particularly glycogen and protein component as well as alkaline phosphatase (ALPase) and adenosine triphosphatase (ATPase) in the cells lining the olfactory epithelium of Catla catla. The intense reaction of silver stain was well-marked in the primary receptor cells and in the synaptic contacts between primary and secondary receptor cells. The maximum intensity of reaction of acid and neutral mucin as well as protein, was discernible in the secretory and non-secretory mucous cells. Various shades of glycogen were detected in the receptor cells, stratified epithelial cells and mucous cells. Concentration of ALPase was found to be associated with central core along with basement membrane. Acute ATPase activity was evidenced in the receptor cells, stratified epithelial cells and basal cells of olfactory epithelium and nerve fibers in the central core. Histochemical reactions in the various cells on the olfactory epithelium were discussed with the functional importance of the fish concerned.
Keywords
Cyprinidae
Olfactory organ
Neurons
Mucopolysaccharides
Glycogen
Protein
Enzymes.
Article Details
License
The authors transfer the copyrights of their papers to the Iranian Society of Ichthyology. However, the information could be used in accordance with the Creative Commons licence (Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
How to Cite
GHOSH, S. K., & CHAKRABARTI, P. (2015). Histochemical characterization of the olfactory epithelium of an Indian major carp, Catla catla (Hamilton, 1822). Iranian Journal of Ichthyology, 2(1), 43–52. https://doi.org/10.22034/iji.v2i1.14
References
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Agrawal, S.K.; Banerjee, T.K. & Mittal, A.K. 1979. Enzyme in the epidermis of a freshwater teleost Barbus sophore (Cyprinidae, Pisces): a histochemical investigation. Mikroskopie 35: 258-264.
Andres, K.H.; 1966. Der Feinball der Regio olfactoria von makro smatikern. Zeitschrift für Zellforschung und mikroskopische Anatomie 69: 140-154.
Andres, K.H.; 1969. Der Olfaktorische Saum der Katze. Zeitschrift für Zellforschung und mikroskopische Anatomie 96: 250-274.
Best, F. 1906. Uber carmine far bug des glycogens and derkerne.
Zeitschrift für wissenschaftliche Mikroskopie und mikroskopische Technik 3 319–322. Bhute, Y.V. & Baile, V.V. 2007. Organization of the olfactory system of the Indian major carp Labeo rohita (Ham.): a scanning and transmission electron microscopy study. Journal of Evolutionary Biochemistry and Physiology 43: 342-349.
Camacho, S.; Ostos-Garrido, M.V.; Domezain, A. & Carmona, R. 2010. Study of the olfactory epithelium in the developing sturgeon characterization of the crypt cells. Chemical Senses 35: 147-156.
Chakarbarti, P. 2005. Histological and histochemical studies on the olfactory rosette of Mugil parsia (Hamilton). Folia Morphologica 64: 41-46.
Chakrabarti, P. & Ghosh, S.K. 2009. Ultrastructural organisation and functional aspects of the olfactory epithelium of Wallago attu (Bleeker). Folia Morphologica 68: 40-44.
Chakrabarti, P. & Ghosh, S.K. 2010a. Histoarchitecture and scanning electron microscopic studies of the olfactory epithelium in the exotic fish Puntius javanicus (Bleeker). Archieves of Polish Fisheries 18: 173-177.
Chakrabarti, P. & Ghosh, S.K. 2010b. Histological and scanning electron microscopical study of the olfactory epithelium of Indian major carp, Catla catla (Hamilton). Folia Morphologica 69: 24-29.
Chakrabarti, P. & Ghosh, S.K. 2013. Histological and histochemical studies of the olfactory organ in bagrid catfish Rita rita (Hamilton, 1822). Journal of Biological Sciences 13: 242-249.
Cuschieri, A. 1974. Enzyme histochemistry of the olfactory mucosa and vomeronasal organ in the mouse. Journal of Anatomy 118: 477-189.
Datta Munshi, J.S. & Singh, S.P. 1975. Histochemical observations on the olfactory mucosa of the Indian green snake headed fish, Channa puncatata (Bloch). Proceedings of the Zoological Society of India? 28: 1-13.
Ghosh, S.K. & Chakrabarti, P. 2012. Histological organization and microarchitecture of various cells lining the olfactory epithelium of Rita rita (Hamilton, 1822) (Siluriformes: Bagridae). Biological Letters 49: 35-42.
Ghosh, S.K. & Chakrabarti, P. 2013. Topological organization and functional aspects of the olfactory epithelium of whipfin silver biddy Gerres filamentosus (Cuvier 1829). Pakistan Journal of Biological Sciences 16: 245-250.
Ghosh, S.K. & Chakrabarti, P. 2014a. Structural characterization of the olfactory epithelium of freshwater olive barb, Puntius sarana (Hamilton, 1822). International Journal of Aquatic Biology 2: 147-154.
Ghosh, S.K. & Chakrabarti, P. 2014b. Histochemical investigations on the olfactory epithelium of the freshwater minor carp, Labeo bata (Hamilton, 1822). Bioinfo Aquatic Ecosystem 3: 54-58.
Gomori, G. 1951. Alkaline phosphatase of cell nuclei. Journal of Laboratory and Clinical Medicine 37: 526.
Hansen, A. & Zeiske, E. 1998. The peripheral olfactory organ of the zebrafish, Danio rerio: an ultrastructural study. Chemical Senses 23: 39-48.
Hara, T.J. 1994. The diversity of chemical stimulation in fish olfaction and gestation. In: Singh, H.R. (eds.), Advances in fish biology and fisheries, Vol. 1, Hindustan Publishing Corporation, Delhi, pp. 167-184.
Hara, T.J. & Zielinski, B. 1989. Structural and functional development of the olfactory organ in teleosts. Transactions of the American Fisheries Society 118: 183-194.
Hernadi, L. 1993. Fine structural characterization of the olfactory epithelium and its response to divalent cations Cd2+ in the fish Alburnus alburnus (Teleostei, Cyprinidae): a scanning and transmission electron microscopic study. Neurobiology 1: 11-31.
Kuciel, M.; Żuwala, K. & Jakubowski, M. 2011. A new type of fish olfactory organ structure in Periophthalmus barbarous (Oxudercinae). Acta Zoologica 92: 276-280.
Kumari, K. 2008. Morphology and morphometry of the olfactory rosette of a teleostean fish: Catla catla (Ham.). Our Nature 6: 30-37.
Mana, R.R. & Kawamura, G. 2002. A comparative study on morphological differences in the olfactory system of red sea bream (Pagrus major) and black sea bream (Acanthopagrus schlegeli) from wild and cultured stocks. Aquaculture 22: 53-64.
Mandal D.K.; Roy, D. & Ghosh, L. 2005. Structural organization of the olfactory epithelium of a spotted snakehead fish, Channa punctatus. Acta Ichthyologica et Piscatoria 35: 45-50.
Marsland, T.A.; Glees, P. & Erikson, L.B. 1954. Modification of the Glees Silver impregnation for paraffin sections. Journal of Neuropathology & Experimental Neurology 13: 587-591.
Mazia, D.; Brewer, P.A. & Alfert, M. 1953. The cytochemical staining and measurement of protein with mercuric bromphenol blue. Biology Bulletin 104: 57.
Moog, F. 1946. The physiological significance of phosphomonoesterases. Biological Reviews 21: 41-59.
Mowry, R.W. 1956. Alcian blue technique for the histochemical study of acidic carbohydrates. Journal of Histochemistry and Cytochemistry 4: 403.
Padykula, H.A. & Herman, E. 1955. The specificity of histochemical method for adenosine triphosphatase. Journal of Histochemistry and Cytochemistry 3: 170-175.
Rahmani, A.R. & Khan, S.M. 1980. Histology of the olfactory epithelium and the accessory nasal sacs of an anabantoid fish, Anabas testudineus (Bloch). Archives de Biologie 91: 397-411.
Sawad, A.A.; Hussein, S.A. & Al-Nusear, A.N. 2006. Morphological and histological study for some sensory organs in common carp (Cyprinus carpio L.). Marina Mesopotamica 22: 197-207.
Shantha, T.R. & Nakajima, Y. 1970. Histological and histochemical studies on the rhesus monkey (Macaca mulatta) olfactory mucosa. Zeitschrift für Zellforschung und mikroskopische Anatomie 103: 291-319.
Singh, S.P. 1977. Functional anatomy of olfactory organs in some marine teleosts. Zoologischer Anzeiger 56: 441-444.
Sinha, G.M. 1975. A histochemical study of the mucous cells in the buccopharyngeal region of four Indian freshwater fishes in relation to their origin, development, occurrence and probable functions. Acta Histochemica 53: 217-223.
Theisen, B. 1972. Ultrstructure of the olfactory epithelium in the Australian lungfish, Neoceratodus forsteri, Acta Zoologica 53: 205-218.
Weinreb, E.L. & Bilstad, N.M. 1955. Histology of the digestive tract and adjacent structures of a rainbow trout, Salmo gairdneri irideus. Copeia 1955(3): 194-204.
Yamamoto, M. & Ueda, K. 1978. Comparative morphology of fish olfactory epithelium-III. Cypriniformes. Bulletin of the Japanese Society of Scientific Fisheries 44: 1201-1206.
References
Agrawal, S.K.; Banerjee, T.K. & Mittal, A.K. 1979. Enzyme in the epidermis of a freshwater teleost Barbus sophore (Cyprinidae, Pisces): a histochemical investigation. Mikroskopie 35: 258-264.
Andres, K.H.; 1966. Der Feinball der Regio olfactoria von makro smatikern. Zeitschrift für Zellforschung und mikroskopische Anatomie 69: 140-154.
Andres, K.H.; 1969. Der Olfaktorische Saum der Katze. Zeitschrift für Zellforschung und mikroskopische Anatomie 96: 250-274.
Best, F. 1906. Uber carmine far bug des glycogens and derkerne.
Zeitschrift für wissenschaftliche Mikroskopie und mikroskopische Technik 3 319–322. Bhute, Y.V. & Baile, V.V. 2007. Organization of the olfactory system of the Indian major carp Labeo rohita (Ham.): a scanning and transmission electron microscopy study. Journal of Evolutionary Biochemistry and Physiology 43: 342-349.
Camacho, S.; Ostos-Garrido, M.V.; Domezain, A. & Carmona, R. 2010. Study of the olfactory epithelium in the developing sturgeon characterization of the crypt cells. Chemical Senses 35: 147-156.
Chakarbarti, P. 2005. Histological and histochemical studies on the olfactory rosette of Mugil parsia (Hamilton). Folia Morphologica 64: 41-46.
Chakrabarti, P. & Ghosh, S.K. 2009. Ultrastructural organisation and functional aspects of the olfactory epithelium of Wallago attu (Bleeker). Folia Morphologica 68: 40-44.
Chakrabarti, P. & Ghosh, S.K. 2010a. Histoarchitecture and scanning electron microscopic studies of the olfactory epithelium in the exotic fish Puntius javanicus (Bleeker). Archieves of Polish Fisheries 18: 173-177.
Chakrabarti, P. & Ghosh, S.K. 2010b. Histological and scanning electron microscopical study of the olfactory epithelium of Indian major carp, Catla catla (Hamilton). Folia Morphologica 69: 24-29.
Chakrabarti, P. & Ghosh, S.K. 2013. Histological and histochemical studies of the olfactory organ in bagrid catfish Rita rita (Hamilton, 1822). Journal of Biological Sciences 13: 242-249.
Cuschieri, A. 1974. Enzyme histochemistry of the olfactory mucosa and vomeronasal organ in the mouse. Journal of Anatomy 118: 477-189.
Datta Munshi, J.S. & Singh, S.P. 1975. Histochemical observations on the olfactory mucosa of the Indian green snake headed fish, Channa puncatata (Bloch). Proceedings of the Zoological Society of India? 28: 1-13.
Ghosh, S.K. & Chakrabarti, P. 2012. Histological organization and microarchitecture of various cells lining the olfactory epithelium of Rita rita (Hamilton, 1822) (Siluriformes: Bagridae). Biological Letters 49: 35-42.
Ghosh, S.K. & Chakrabarti, P. 2013. Topological organization and functional aspects of the olfactory epithelium of whipfin silver biddy Gerres filamentosus (Cuvier 1829). Pakistan Journal of Biological Sciences 16: 245-250.
Ghosh, S.K. & Chakrabarti, P. 2014a. Structural characterization of the olfactory epithelium of freshwater olive barb, Puntius sarana (Hamilton, 1822). International Journal of Aquatic Biology 2: 147-154.
Ghosh, S.K. & Chakrabarti, P. 2014b. Histochemical investigations on the olfactory epithelium of the freshwater minor carp, Labeo bata (Hamilton, 1822). Bioinfo Aquatic Ecosystem 3: 54-58.
Gomori, G. 1951. Alkaline phosphatase of cell nuclei. Journal of Laboratory and Clinical Medicine 37: 526.
Hansen, A. & Zeiske, E. 1998. The peripheral olfactory organ of the zebrafish, Danio rerio: an ultrastructural study. Chemical Senses 23: 39-48.
Hara, T.J. 1994. The diversity of chemical stimulation in fish olfaction and gestation. In: Singh, H.R. (eds.), Advances in fish biology and fisheries, Vol. 1, Hindustan Publishing Corporation, Delhi, pp. 167-184.
Hara, T.J. & Zielinski, B. 1989. Structural and functional development of the olfactory organ in teleosts. Transactions of the American Fisheries Society 118: 183-194.
Hernadi, L. 1993. Fine structural characterization of the olfactory epithelium and its response to divalent cations Cd2+ in the fish Alburnus alburnus (Teleostei, Cyprinidae): a scanning and transmission electron microscopic study. Neurobiology 1: 11-31.
Kuciel, M.; Żuwala, K. & Jakubowski, M. 2011. A new type of fish olfactory organ structure in Periophthalmus barbarous (Oxudercinae). Acta Zoologica 92: 276-280.
Kumari, K. 2008. Morphology and morphometry of the olfactory rosette of a teleostean fish: Catla catla (Ham.). Our Nature 6: 30-37.
Mana, R.R. & Kawamura, G. 2002. A comparative study on morphological differences in the olfactory system of red sea bream (Pagrus major) and black sea bream (Acanthopagrus schlegeli) from wild and cultured stocks. Aquaculture 22: 53-64.
Mandal D.K.; Roy, D. & Ghosh, L. 2005. Structural organization of the olfactory epithelium of a spotted snakehead fish, Channa punctatus. Acta Ichthyologica et Piscatoria 35: 45-50.
Marsland, T.A.; Glees, P. & Erikson, L.B. 1954. Modification of the Glees Silver impregnation for paraffin sections. Journal of Neuropathology & Experimental Neurology 13: 587-591.
Mazia, D.; Brewer, P.A. & Alfert, M. 1953. The cytochemical staining and measurement of protein with mercuric bromphenol blue. Biology Bulletin 104: 57.
Moog, F. 1946. The physiological significance of phosphomonoesterases. Biological Reviews 21: 41-59.
Mowry, R.W. 1956. Alcian blue technique for the histochemical study of acidic carbohydrates. Journal of Histochemistry and Cytochemistry 4: 403.
Padykula, H.A. & Herman, E. 1955. The specificity of histochemical method for adenosine triphosphatase. Journal of Histochemistry and Cytochemistry 3: 170-175.
Rahmani, A.R. & Khan, S.M. 1980. Histology of the olfactory epithelium and the accessory nasal sacs of an anabantoid fish, Anabas testudineus (Bloch). Archives de Biologie 91: 397-411.
Sawad, A.A.; Hussein, S.A. & Al-Nusear, A.N. 2006. Morphological and histological study for some sensory organs in common carp (Cyprinus carpio L.). Marina Mesopotamica 22: 197-207.
Shantha, T.R. & Nakajima, Y. 1970. Histological and histochemical studies on the rhesus monkey (Macaca mulatta) olfactory mucosa. Zeitschrift für Zellforschung und mikroskopische Anatomie 103: 291-319.
Singh, S.P. 1977. Functional anatomy of olfactory organs in some marine teleosts. Zoologischer Anzeiger 56: 441-444.
Sinha, G.M. 1975. A histochemical study of the mucous cells in the buccopharyngeal region of four Indian freshwater fishes in relation to their origin, development, occurrence and probable functions. Acta Histochemica 53: 217-223.
Theisen, B. 1972. Ultrstructure of the olfactory epithelium in the Australian lungfish, Neoceratodus forsteri, Acta Zoologica 53: 205-218.
Weinreb, E.L. & Bilstad, N.M. 1955. Histology of the digestive tract and adjacent structures of a rainbow trout, Salmo gairdneri irideus. Copeia 1955(3): 194-204.
Yamamoto, M. & Ueda, K. 1978. Comparative morphology of fish olfactory epithelium-III. Cypriniformes. Bulletin of the Japanese Society of Scientific Fisheries 44: 1201-1206.
Andres, K.H.; 1966. Der Feinball der Regio olfactoria von makro smatikern. Zeitschrift für Zellforschung und mikroskopische Anatomie 69: 140-154.
Andres, K.H.; 1969. Der Olfaktorische Saum der Katze. Zeitschrift für Zellforschung und mikroskopische Anatomie 96: 250-274.
Best, F. 1906. Uber carmine far bug des glycogens and derkerne.
Zeitschrift für wissenschaftliche Mikroskopie und mikroskopische Technik 3 319–322. Bhute, Y.V. & Baile, V.V. 2007. Organization of the olfactory system of the Indian major carp Labeo rohita (Ham.): a scanning and transmission electron microscopy study. Journal of Evolutionary Biochemistry and Physiology 43: 342-349.
Camacho, S.; Ostos-Garrido, M.V.; Domezain, A. & Carmona, R. 2010. Study of the olfactory epithelium in the developing sturgeon characterization of the crypt cells. Chemical Senses 35: 147-156.
Chakarbarti, P. 2005. Histological and histochemical studies on the olfactory rosette of Mugil parsia (Hamilton). Folia Morphologica 64: 41-46.
Chakrabarti, P. & Ghosh, S.K. 2009. Ultrastructural organisation and functional aspects of the olfactory epithelium of Wallago attu (Bleeker). Folia Morphologica 68: 40-44.
Chakrabarti, P. & Ghosh, S.K. 2010a. Histoarchitecture and scanning electron microscopic studies of the olfactory epithelium in the exotic fish Puntius javanicus (Bleeker). Archieves of Polish Fisheries 18: 173-177.
Chakrabarti, P. & Ghosh, S.K. 2010b. Histological and scanning electron microscopical study of the olfactory epithelium of Indian major carp, Catla catla (Hamilton). Folia Morphologica 69: 24-29.
Chakrabarti, P. & Ghosh, S.K. 2013. Histological and histochemical studies of the olfactory organ in bagrid catfish Rita rita (Hamilton, 1822). Journal of Biological Sciences 13: 242-249.
Cuschieri, A. 1974. Enzyme histochemistry of the olfactory mucosa and vomeronasal organ in the mouse. Journal of Anatomy 118: 477-189.
Datta Munshi, J.S. & Singh, S.P. 1975. Histochemical observations on the olfactory mucosa of the Indian green snake headed fish, Channa puncatata (Bloch). Proceedings of the Zoological Society of India? 28: 1-13.
Ghosh, S.K. & Chakrabarti, P. 2012. Histological organization and microarchitecture of various cells lining the olfactory epithelium of Rita rita (Hamilton, 1822) (Siluriformes: Bagridae). Biological Letters 49: 35-42.
Ghosh, S.K. & Chakrabarti, P. 2013. Topological organization and functional aspects of the olfactory epithelium of whipfin silver biddy Gerres filamentosus (Cuvier 1829). Pakistan Journal of Biological Sciences 16: 245-250.
Ghosh, S.K. & Chakrabarti, P. 2014a. Structural characterization of the olfactory epithelium of freshwater olive barb, Puntius sarana (Hamilton, 1822). International Journal of Aquatic Biology 2: 147-154.
Ghosh, S.K. & Chakrabarti, P. 2014b. Histochemical investigations on the olfactory epithelium of the freshwater minor carp, Labeo bata (Hamilton, 1822). Bioinfo Aquatic Ecosystem 3: 54-58.
Gomori, G. 1951. Alkaline phosphatase of cell nuclei. Journal of Laboratory and Clinical Medicine 37: 526.
Hansen, A. & Zeiske, E. 1998. The peripheral olfactory organ of the zebrafish, Danio rerio: an ultrastructural study. Chemical Senses 23: 39-48.
Hara, T.J. 1994. The diversity of chemical stimulation in fish olfaction and gestation. In: Singh, H.R. (eds.), Advances in fish biology and fisheries, Vol. 1, Hindustan Publishing Corporation, Delhi, pp. 167-184.
Hara, T.J. & Zielinski, B. 1989. Structural and functional development of the olfactory organ in teleosts. Transactions of the American Fisheries Society 118: 183-194.
Hernadi, L. 1993. Fine structural characterization of the olfactory epithelium and its response to divalent cations Cd2+ in the fish Alburnus alburnus (Teleostei, Cyprinidae): a scanning and transmission electron microscopic study. Neurobiology 1: 11-31.
Kuciel, M.; Żuwala, K. & Jakubowski, M. 2011. A new type of fish olfactory organ structure in Periophthalmus barbarous (Oxudercinae). Acta Zoologica 92: 276-280.
Kumari, K. 2008. Morphology and morphometry of the olfactory rosette of a teleostean fish: Catla catla (Ham.). Our Nature 6: 30-37.
Mana, R.R. & Kawamura, G. 2002. A comparative study on morphological differences in the olfactory system of red sea bream (Pagrus major) and black sea bream (Acanthopagrus schlegeli) from wild and cultured stocks. Aquaculture 22: 53-64.
Mandal D.K.; Roy, D. & Ghosh, L. 2005. Structural organization of the olfactory epithelium of a spotted snakehead fish, Channa punctatus. Acta Ichthyologica et Piscatoria 35: 45-50.
Marsland, T.A.; Glees, P. & Erikson, L.B. 1954. Modification of the Glees Silver impregnation for paraffin sections. Journal of Neuropathology & Experimental Neurology 13: 587-591.
Mazia, D.; Brewer, P.A. & Alfert, M. 1953. The cytochemical staining and measurement of protein with mercuric bromphenol blue. Biology Bulletin 104: 57.
Moog, F. 1946. The physiological significance of phosphomonoesterases. Biological Reviews 21: 41-59.
Mowry, R.W. 1956. Alcian blue technique for the histochemical study of acidic carbohydrates. Journal of Histochemistry and Cytochemistry 4: 403.
Padykula, H.A. & Herman, E. 1955. The specificity of histochemical method for adenosine triphosphatase. Journal of Histochemistry and Cytochemistry 3: 170-175.
Rahmani, A.R. & Khan, S.M. 1980. Histology of the olfactory epithelium and the accessory nasal sacs of an anabantoid fish, Anabas testudineus (Bloch). Archives de Biologie 91: 397-411.
Sawad, A.A.; Hussein, S.A. & Al-Nusear, A.N. 2006. Morphological and histological study for some sensory organs in common carp (Cyprinus carpio L.). Marina Mesopotamica 22: 197-207.
Shantha, T.R. & Nakajima, Y. 1970. Histological and histochemical studies on the rhesus monkey (Macaca mulatta) olfactory mucosa. Zeitschrift für Zellforschung und mikroskopische Anatomie 103: 291-319.
Singh, S.P. 1977. Functional anatomy of olfactory organs in some marine teleosts. Zoologischer Anzeiger 56: 441-444.
Sinha, G.M. 1975. A histochemical study of the mucous cells in the buccopharyngeal region of four Indian freshwater fishes in relation to their origin, development, occurrence and probable functions. Acta Histochemica 53: 217-223.
Theisen, B. 1972. Ultrstructure of the olfactory epithelium in the Australian lungfish, Neoceratodus forsteri, Acta Zoologica 53: 205-218.
Weinreb, E.L. & Bilstad, N.M. 1955. Histology of the digestive tract and adjacent structures of a rainbow trout, Salmo gairdneri irideus. Copeia 1955(3): 194-204.
Yamamoto, M. & Ueda, K. 1978. Comparative morphology of fish olfactory epithelium-III. Cypriniformes. Bulletin of the Japanese Society of Scientific Fisheries 44: 1201-1206.